Invitrogen

HDAC6 Polyclonal Antibody

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HDAC6 Antibody in Western Blot (WB) — Western Blot analysis of HDAC6 in extracts of various cell lines using HDAC6 Polyclonal Antibody (Product # PA5-95908) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

HDAC6 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of HDAC6 in paraffin-embedded rat kidney using HDAC6 Polyclonal Antibody (Product # PA5-95908) at a dilution of 1:100.

HDAC6 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of HDAC6 in paraffin-embedded human stomach using HDAC6 Polyclonal Antibody (Product # PA5-95908) at a dilution of 1:100.

HDAC6 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of HDAC6 in paraffin-embedded mouse kidney using HDAC6 Polyclonal Antibody (Product # PA5-95908) at a dilution of 1:100.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500 - 1:2000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50 - 1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human HDAC6.

Conjugate

Unconjugated

Form

Liquid

Concentration

1.41 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2807710

Product Specific Information

Immunogen sequence: MTSTGQDSTT TRQRRSRQNP QSPPQDSSVT SKRNIKKGAV PRSIPNLAEV KKKGKMKKLG QAMEEDLIVG LQGMDLNLEA EALAGTGLVL DEQLNEFHCL WDDSFPEGPE RLHAIKEQLI QEGLLDRCVS FQARFAEKEE LMLVHSLEYI DLMETTQYMN EGELRVLADT YDSVYLHPNS YSCACLASGS VLRLVDAVLG; Positive Samples: K-562, HeLa; Cellular Location: Cell projection, Cytoplasm, Nucleus, Perikaryon, axon, dendrite

Target Information

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ16239; HD6; HDA6; histone deacetylase 5; Histone deacetylase 6; Histone deacetylase mHDA2; protein phosphatase 1, regulatory subunit 90; scurfy candidate 6

Gene Aliases: CPBHM; HD6; Hdac5; HDAC6; JM21; KIAA0901; mHDA2; PPP1R90; Sfc6

UniProt ID: (Human) Q9UBN7, (Mouse) Q9Z2V5

Entrez Gene ID: (Human) 10013, (Rat) 84581, (Mouse) 15185

Molecular Function: deacetylase hydrolase nucleic acid binding oxidoreductase reductase

protein polyubiquitination transcription, DNA-templated protein deacetylation misfolded or incompletely synthesized protein catabolic process intracellular protein transport organelle organization negative regulation of microtubule depolymerization response to toxic substance positive regulation of signal transduction response to organic substance regulation of receptor activity regulation of autophagy positive regulation of epithelial cell migration negative regulation of hydrogen peroxide metabolic process positive regulation of receptor biosynthetic process macroautophagy histone deacetylation regulation of protein stability lysosome localization peptidyl-lysine deacetylation cellular response to topologically incorrect protein regulation of gene expression, epigenetic ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway protein complex disassembly negative regulation of protein complex disassembly regulation of fat cell differentiation negative regulation of proteolysis negative regulation of transcription, DNA-templated negative regulation of oxidoreductase activity mitochondrion localization response to misfolded protein regulation of microtubule-based movement regulation of androgen receptor signaling pathway regulation of establishment of protein localization cellular response to hydrogen peroxide aggresome assembly polyubiquitinated misfolded protein transport Hsp90 deacetylation response to growth factor histone H3 deacetylation cellular response to misfolded protein positive regulation of chaperone-mediated protein complex assembly tubulin deacetylation mitophagy in response to mitochondrial depolarization positive regulation of hydrogen peroxide-mediated programmed cell death regulation of mitophagy regulation of protein phosphorylation response to amphetamine ubiquitin-dependent protein catabolic process metabolic process positive regulation of cell proliferation regulation of mitochondrion organization negative regulation of neuron projection development axonal transport of mitochondrion neuron differentiation cellular response to oxidative stress response to immobilization stress negative regulation of axon extension involved in axon guidance positive regulation of dendrite morphogenesis response to corticosterone positive regulation of synaptic transmission, glutamatergic regulation of microtubule cytoskeleton organization cellular response to parathyroid hormone stimulus response to dexamethasone positive regulation of tubulin deacetylation negative regulation of protein acetylation positive regulation of oxidative stress-induced neuron death regulation of transcription, DNA-templated chromatin modification

Process(es)

core promoter binding actin binding histone deacetylase activity protein binding beta-catenin binding microtubule binding zinc ion binding enzyme binding polyubiquitin binding ubiquitin protein ligase binding NAD-dependent histone deacetylase activity (H3-K14 specific) histone deacetylase binding tubulin deacetylase activity alpha-tubulin binding tau protein binding beta-tubulin binding misfolded protein binding Hsp90 protein binding dynein complex binding hydrolase activity ubiquitin binding histone deacetylase activity (H3-K14 specific) histone deacetylase activity (H3-K9 specific) histone deacetylase activity (H4-K16 specific) metal ion binding NAD-dependent histone deacetylase activity (H3-K9 specific) NAD-dependent histone deacetylase activity (H4-K16 specific) NAD-dependent histone deacetylase activity (H3-K18 specific)