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Invitrogen
HDAC8 Polyclonal Antibody
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Product Details
PA5-79353
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human HDAC8 (18-33aa YIYSPEYVSMCDSLAK).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: Rat Cardiac Muscle: Tissue, A549 whole cell. IHC: Human Lung Cancer Tissue.
Target Information
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC8, isolated from human kidney, is a histone deacetylase that shares homology to other HDACs but has different tissue distribution. HDAC8 is localized to the nucleus and plays a role in the development of a broad range of tissues and in the etiology of cancer.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HD8; Histone deacetylase 8; histone deacetylase-like 1; Protein deacetylase HDAC8; Protein decrotonylase HDAC8; unnamed protein product
Gene Aliases: 2610007D20Rik; CDA07; CDLS5; HD8; HDAC8; HDACL1; KDAC8; MRXS6; RGD1562895; RPD3; WTS
UniProt ID: (Human) Q9BY41, (Rat) B1WC68, (Mouse) Q8VH37
Entrez Gene ID: (Human) 55869, (Rat) 363481, (Mouse) 70315
histone deacetylase activity
protein binding
hydrolase activity
Hsp70 protein binding
protein deacetylase activity
metal ion binding
Hsp90 protein binding
DNA-binding transcription factor binding
histone deacetylase activity, hydrolytic mechanism
protein decrotonylase activity
histone decrotonylase activity
NAD-dependent histone deacetylase activity (H3-K14 specific)
NAD-dependent histone deacetylase activity (H3-K9 specific)
NAD-dependent histone deacetylase activity (H4-K16 specific)
NAD-dependent histone deacetylase activity (H3-K18 specific)
transcription factor binding
histone deacetylase activity (H3-K14 specific)
histone deacetylase activity (H3-K9 specific)
histone deacetylase activity (H4-K16 specific)
transcription, DNA-templated
regulation of transcription, DNA-templated
sister chromatid cohesion
histone H3-K9 modification
histone H3 deacetylation
histone H4 deacetylation
regulation of cohesin loading
negative regulation of transcription from RNA polymerase II promoter
chromatin organization
mitotic sister chromatid cohesion
negative regulation of protein ubiquitination
heterochromatin assembly
regulation of protein stability
regulation of telomere maintenance
chromatin modification
histone deacetylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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