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Invitrogen
HDAC9 Polyclonal Antibody
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FIGURE: 1 / 4
HDAC9 Antibody (PA5-23346) in WB
Western blot analysis of HDAC9 in human HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) and mouse RAW cell lysate. Samples were incubated in HDAC9 polyclonal antibody (Product # PA5-23346) using a dilution of 0 µg/mL, 1.0 µg/mL and 0.5 µg/mL respectively followed by a goat anti-rabbit Ig HRP secondary antibody. PicoTect ECL substrate solution was used for this test.
Product Details
PA5-23346
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Human,
Mouse,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A portion of amino acids 100-150 of human HDAC-9.
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is a critical component of transcriptional regulation and the acetylation of nucleosomal histones is a major source of this remodeling. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Several mammalian proteins function as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Conversely, the deacetylation of histones is associated with transcriptional silencing. The histone deacetylases (HDAC) include HDAC1-9. HDAC9 and HDAC9a are two alternatively spliced isoforms of HDAC9. HDAC9a is 132 amino acids shorter than HDAC9, but both isoforms contain the HDAC catalytic domain, remain capable of deacetylase activity and repress myoctye enhancer-binding factor 2-mediated transcription. HDAC9 and HDAC9a are expressed in brain, skeletal muscle, kidney, placenta and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp779K1053; HD7; HD7b; HD9; hdac-9; histone deacetylase 4/5-related protein; Histone deacetylase 7B; Histone deacetylase 9; Histone deacetylase-related protein; MEF-2 interacting transcription repressor (MITR) protein; MEF2-interacting transcription repressor MITR; protein deacetylase; unnamed protein product
Gene Aliases: ARCND4; AV022454; D030072B18Rik; HD7; HD7B; HD9; HDAC; HDAC7; HDAC7B; HDAC9; HDAC9B; HDAC9FL; HDRP; KIAA0744; MITR; mKIAA0744; TWIST
UniProt ID: (Human) Q9UKV0, (Mouse) Q99N13
Entrez Gene ID: (Dog) 482339, (Human) 9734, (Mouse) 79221
transcription corepressor activity
histone deacetylase activity
protein kinase C binding
protein binding
hydrolase activity
histone deacetylase activity (H3-K14 specific)
histone deacetylase activity (H3-K9 specific)
protein deacetylase activity
histone deacetylase activity (H4-K16 specific)
histone deacetylase binding
metal ion binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
DNA-binding transcription factor binding
histone deacetylase activity, hydrolytic mechanism
transcription factor binding
NAD-dependent histone deacetylase activity (H3-K14 specific)
NAD-dependent histone deacetylase activity (H3-K9 specific)
NAD-dependent histone deacetylase activity (H4-K16 specific)
repressing transcription factor binding
NAD-dependent histone deacetylase activity (H3-K18 specific)
histone modifying enzyme
negative regulation of transcription from RNA polymerase II promoter
negative regulation of cytokine production
chromatin organization
inflammatory response
heart development
negative regulation of macromolecule biosynthetic process
B cell differentiation
cellular response to insulin stimulus
regulation of gene expression, epigenetic
B cell activation
cholesterol homeostasis
negative regulation of gene expression, epigenetic
negative regulation of transcription, DNA-templated
regulation of skeletal muscle fiber development
regulation of striated muscle cell differentiation
positive regulation of cell migration involved in sprouting angiogenesis
transcription, DNA-templated
regulation of transcription, DNA-templated
nervous system development
chromatin modification
histone deacetylation
peptidyl-lysine deacetylation
negative regulation of striated muscle tissue development
histone H3 deacetylation
histone H4 deacetylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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