Proteintech

HDGFRP3 Polyclonal Antibody

View all (7) HDGFRP3 antibodies

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HDGFRP3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MCF-7 cells, using HDGFRP3 antibody 12380-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

HDGFRP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate cancer using 12380-1-AP (HDGFRP3 antibody) at dilution of 1:50 (under 10x lens).

HDGFRP3 Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 12380-1-AP (HDGFRP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

HDGFRP3 Antibody in Western Blot (WB) — Y79 cells were subjected to SDS PAGE followed by western blot with 12380-1-AP (HDGFRP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

HDGFRP3 Antibody in Immunoprecipitation (IP) — IP result of anti-HDGFRP3 (IP:12380-1-AP, 3ug; Detection:12380-1-AP 1:300) with mouse testis tissue lysate 4800ug.

Product Details

12380-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:20-1:200	View 1 publication 1 publication
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HDGFRP3 Fusion Protein Ag3046 (1-203 aa encoded by BC015483) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.24 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MARPRPREY KAGDLVFAKM KGYPHWPARI DELPEGAVKP PANKYPIFFF GTHETAFLGP KDLFPYKEYK DKFGKSNKRK GFNEGLWEIE NNPGVKFTGY QAIQQQSSSE TEGEGGNTAD ASSEEEGDRV EEDGKGKRKN EKAGSKRKKS YTSKKSSKQS RKSPGDEDDK DCKEEENKSS SEGGDAGNDT RNTTSDLQKT SEGT (1-203 aa encoded by BC015483)

Target Information

HDGFRP3 enhances DNA synthesis and may play a role in cell proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Knockdown of hepatoma-derived growth factor-related protein-3 induces apoptosis of H1299 cells via ROS-dependent and p53-independent NF-κB activation.

Biochemical and biophysical research communications

Yun HS,Baek JH,Yim JH,Lee SJ,Lee CW,Song JY,Um HD,Park JK,Park IC,Hwang SG

Fri Jul 11 00:00:00 EDT 2014

Depletion of hepatoma-derived growth factor-related protein-3 induces apoptotic sensitization of radioresistant A549 cells via reactive oxygen species-dependent p53 activation.

Biochemical and biophysical research communications

Yun HS,Hong EH,Lee SJ,Baek JH,Lee CW,Yim JH,Um HD,Hwang SG

Fri Sep 27 00:00:00 EDT 2013

Depletion of hepatoma-derived growth factor-related protein-3 induces apoptotic sensitization of radioresistant A549 cells via reactive oxygen species-dependent p53 activation.

Biochemical and biophysical research communications

Yun HS,Hong EH,Lee SJ,Baek JH,Lee CW,Yim JH,Um HD,Hwang SG

Fri Sep 27 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: HDGF-2; Hepatoma-derived growth factor 2; Hepatoma-derived growth factor-related protein 3; HRP-3

Gene Aliases: 2700022B06Rik; CGI-142; HDGF-2; HDGF2; HDGFL3; HDGFRP3; HRP-3; Hrp3

UniProt ID: (Human) Q9Y3E1, (Mouse) Q9JMG7, (Rat) Q923W4

Entrez Gene ID: (Human) 50810, (Mouse) 29877, (Rat) 252941

Molecular Function: transcription cofactor

negative regulation of microtubule depolymerization cell proliferation neuron projection development microtubule polymerization

Process(es)

microtubule binding growth factor activity tubulin binding chromatin binding