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Invitrogen
HES5 Recombinant Rabbit Monoclonal Antibody (R4G8)
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FIGURE: 1 / 9
HES5 Antibody (MA572216) in IHC (P)
Immunohistochemistry analysis of HES5 on paraffin-embedded Human Endometrial Cancer tissue. Antigen retrieval by boiling in sodium citrate buffer (pH 6.0) for 15 min. Samples were incubated with HES5 Recombinant Monoclonal (Product # MA572216) using a dilution at 1:200 overnight at 4°C. Followed by DAB staining.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA572216
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
R4G8
Immunogen
KLH conjugated synthetic peptide derived from human HES5.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.45 mg/mL
Amount
45 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Hairy and Enhancer-of-split homologues 5 (HES5) is a helix-loop-helix transcriptional factor that negatively regulates cell differentiation during embryogenesis. It contains one basic helix-loop-helix (bHLH) domain and one Orange domain. HES5 is reported to be a down stream effector of the Notch Signaling Pathway and is expressed in advanced ovarian serous adenocarcinomas as well as in cervical squamous carcinoma. Reports also suggest that HES5 is abundantly expressed in osteoarthritis along with Notch1 and Jagged1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: class B basic helix-loop-helix protein 38; hairy and enhancer of split 5; Notch pathway transcription factor; RP3-395M20.5; unnamed protein product
Gene Aliases: bHLHb38
UniProt ID: (Human) Q5TA89, (Mouse) P70120, (Rat) Q03062
Entrez Gene ID: (Human) 388585, (Mouse) 15208, (Rat) 79225
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
protein dimerization activity
sequence-specific double-stranded DNA binding
chromatin binding
double-stranded DNA binding
protein binding
negative regulation of transcription from RNA polymerase II promoter
chondrocyte differentiation
regulation of transcription, DNA-templated
cell adhesion
Notch signaling pathway
smoothened signaling pathway
brain development
positive regulation of cell proliferation
anterior/posterior pattern specification
oligodendrocyte development
telencephalon development
glial cell fate commitment
forebrain radial glial cell differentiation
neural tube development
central nervous system myelination
neuron differentiation
positive regulation of BMP signaling pathway
regulation of myelination
camera-type eye development
regulation of cell differentiation
negative regulation of auditory receptor cell differentiation
negative regulation of neuron differentiation
positive regulation of Notch signaling pathway
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JAK-STAT cascade
cell maturation
animal organ development
positive regulation of smooth muscle cell proliferation
astrocyte differentiation
negative regulation of astrocyte differentiation
negative regulation of oligodendrocyte differentiation
system development
regulation of epithelial cell proliferation
regulation of neurogenesis
cartilage development
inner ear receptor stereocilium organization
protein-containing complex assembly
comma-shaped body morphogenesis
S-shaped body morphogenesis
specification of loop of Henle identity
metanephric nephron tubule morphogenesis
establishment of epithelial cell polarity
neuronal stem cell population maintenance
negative regulation of stem cell differentiation
negative regulation of pro-B cell differentiation
negative regulation of forebrain neuron differentiation
negative regulation of inner ear receptor cell differentiation
negative regulation of cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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