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Invitrogen
HES6 Polyclonal Antibody
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FIGURE: 1 / 2
HES6 Antibody (PA5-103203) in ICC/IF
Immunocytochemistry analysis of HES6 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with HES6 polyclonal antibody (Product # PA5-103203) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103203
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human HES6(Accession Q96HZ4), corresponding to amino acid residues M1-K36.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total HES6.
Target Information
The HES family members form a complex with TLE, the mammalian homologue of Groucho, and this interaction is mediated by the carboxy terminal WRPW motif of the HES proteins. The HES/TLE complex functions by directly binding to DNA, instead of interfering with activator proteins. Most HES family members, including HES1 and HES5, preferentially bind to the N box (CACNAG) as opposed to the E box (CANNTG). HES2 binds to both N and E box sites, while HES6 does not bind DNA. Rather, HES6 inhibits HES1 activity, thereby promoting transcription. HES1 and HES2 are expressed in a variety of adult and embryonic tissues. HES3 is expressed exclusively in cerebellar Purkinje cells, and HES5 is found solely in the nervous system. HES6 is produced in brain as well as in the limb buds of developing embryos.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLH-WRPW transcription factor HES-6; bHLHb41; C-HAIRY1; Class B basic helix-loop-helix protein 41; Hairy and enhancer of split 6; Transcription cofactor HES-6; unnamed protein product
Gene Aliases: BHLHB41; bHLHc23; C-HAIRY1; HES-6; HES6
UniProt ID: (Human) Q96HZ4
Entrez Gene ID: (Human) 55502, (Rat) 316626
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein dimerization activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
transcription regulator inhibitor activity
sequence-specific double-stranded DNA binding
transcription cofactor activity
basic helix-loop-helix transcription factor
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
nervous system development
anterior/posterior pattern specification
cell differentiation
positive regulation of transcription from RNA polymerase II promoter
regulation of neurogenesis
transcription, DNA-templated
transcription from RNA polymerase II promoter
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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