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HEYL Antibody (PA5-116766) in WB
Western blot analysis of HEYL in various lysates. Samples were incubated with HEYL Polyclonal antibody (Product # PA5-116766) using a dilution of 1:2,500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-116766
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human HEYL (NP_0553862)
Conjugate
Form
Liquid
Concentration
2.77 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2901397
Product Specific Information
Positive Samples: A-549, BxPC-3^M
^M
Immunogen sequence: MKRPKEPSGS DGESDGPIDV GQEGQLSQMA RPLSTPSSSQ MQARKKHRGI IEKRRRDRIN SSLSELRRLV PTAFEKQGSS KLEKAEVLQM TVDHLKMLHA
Target Information
This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The sequence of the encoded protein contains a conserved bHLH and orange domain, but its YRPW motif has diverged from other HESR family members. It is thought to be an effector of Notch signaling and a regulator of cell fate decisions. Alternatively spliced transcript variants have been found, but their biological validity has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHb33; Class B basic helix-loop-helix protein 33; hairy and enhance of split related 3; Hairy and enhancer of split-related protein 3; Hairy-related transcription factor 3; hairy/enhancer-of-split related with YRPW motif 3; Hairy/enhancer-of-split related with YRPW motif-like protein; hes-related family bHLH transcription factor with YRPW motif-like; HEY-like protein; hHeyL; hHRT3; HRT-3; mHRT3
Gene Aliases: BHLHB33; HESR3; HEY3; HEYL; HRT3
UniProt ID: (Human) Q9NQ87, (Mouse) Q9DBX7
Entrez Gene ID: (Human) 26508, (Mouse) 56198
RNA polymerase II core promoter sequence-specific DNA binding
transcription factor activity, RNA polymerase II core promoter sequence-specific
transcription factor activity, protein binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription corepressor activity
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
protein binding
transcription factor binding
microsatellite binding
protein homodimerization activity
protein heterodimerization activity
AF-1 domain binding
DNA binding
sequence-specific DNA binding
protein dimerization activity
basic helix-loop-helix transcription factor
negative regulation of transcription from RNA polymerase II promoter
outflow tract morphogenesis
atrioventricular valve morphogenesis
pulmonary valve morphogenesis
epithelial to mesenchymal transition involved in endocardial cushion formation
endocardial cushion morphogenesis
cardiac ventricle morphogenesis
transcription from RNA polymerase II promoter
Notch signaling pathway
mesenchymal cell development
glomerulus development
skeletal muscle cell differentiation
positive regulation of neuron differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cardiac epithelial to mesenchymal transition
ventricular septum morphogenesis
negative regulation of androgen receptor signaling pathway
cellular response to BMP stimulus
proximal tubule development
negative regulation of androgen receptor activity
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
multicellular organism development
peripheral nervous system development
negative regulation of gene expression
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