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Invitrogen
HFE Polyclonal Antibody
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FIGURE: 1 / 1
HFE Antibody (PA5-88983) in WB
Western blot analysis of HFE in extracts of various cell lines. Samples were incubated with HFE Polyclonal antibody (Product # PA5-88983) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product Details
PA5-88983
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 80-306 of human HFE (NP_0004011)
Conjugate
Form
Liquid
Concentration
1.40 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: SSQMWLQLSQ SLKGWDHMFT VDFWTIMENH NHSKESHTLQ VILGCEMQED NSTEGYWKYG YDGQDHLEFC PDTLDWRAAE PRAWPTKLEW ERHKIRARQN RAYLERDCPA QLQQLLELGR GVLDQQVPPL VKVTHHVTSS VTTLRCRALN YYPQNITMKW LKDKQPMDAK EFEPKDVLPN GDGTYQGWIT LAVPPGEEQR YTCQVEHPGL DQPLIVIWEP SPSGTLV; Positive Samples: Mouse liver, Mouse heart; Cellular Location: Cell membrane, Single-pass type I membrane protein
Target Information
The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin. It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Hereditary hemochromatosis protein; hereditary hemochromatosis protein HLA-H; Hereditary hemochromatosis protein homolog; HFE 1; high Fe; HLA H; HLA-H; MGC10379; MGC103790; MGC:150812; MHC class I-like protein HFE
Gene Aliases: HFE; HFE1; HH; HLA-H; HLAH; MR2; MVCD7; TFQTL2
UniProt ID: (Human) Q30201, (Mouse) P70387
Entrez Gene ID: (Human) 3077, (Mouse) 15216
antigen processing and presentation of peptide antigen via MHC class I
negative regulation of T cell antigen processing and presentation
negative regulation of T cell cytokine production
protein complex assembly
cellular iron ion homeostasis
acute-phase response
immune response
female pregnancy
response to iron ion
cellular response to iron ion starvation
positive regulation of gene expression
positive regulation of pathway-restricted SMAD protein phosphorylation
antigen processing and presentation
BMP signaling pathway
positive regulation of protein binding
negative regulation of proteasomal ubiquitin-dependent protein catabolic process
hormone biosynthetic process
positive regulation of receptor-mediated endocytosis
iron ion homeostasis
multicellular organismal iron ion homeostasis
cellular response to iron ion
positive regulation of peptide hormone secretion
liver regeneration
iron ion import into cell
negative regulation of receptor binding
positive regulation of receptor binding
positive regulation of ferrous iron import into cell
negative regulation of antigen processing and presentation of endogenous peptide antigen via MHC class I
positive regulation of ferrous iron binding
positive regulation of transferrin receptor binding
response to iron ion starvation
regulation of protein localization to cell surface
negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of receptor activity
positive regulation of receptor activity
negative regulation of CD8-positive, alpha-beta T cell activation
immune system process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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