|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Recombinant, human HGF (rhHGF) expressed in SF 21|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 5% trehalose|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5-1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||15 µg/ml|
|Neutralization (Neu)||0.2-0.6 ug/ml|
|Western Blot (WB)||0.1-0.2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28024 detects HGF from human samples.
PA1-28024 has been successfully used in neutralization, immunohistochemistry (paraffin tissue), Western blot, and ELISA applications.
The PA1-28024 immunogen is recombinant, human HGF (rhHGF) expressed in SF 21.
Hepatocyte Growth Factor (HGF), also known as Hepatopoetin A and Scatter Factor, is a potent mitogen for epithelial cells. HGF has a molecular weight of 82-85 kDa. HGF stimulates the growth of hepatocytes, renal tubular epithelial cells, epidermal keratinocytes, epidermal melanocytes, Mv1Lu (mink lung epithelial cells) and BALB/MK (mouse keratinocytes). HGF inhibits the growth of B6/F1 (mouse melanoma) cells, KB (human squamous carcinoma) cells and HepG2 (human hepatoma) cells. The HGF gene spans approximately 70 kb and consists of 18 exons interrupted by 17 introns. The organization of the human HGF gene is highly homologous to that of human plasminogen. HGF maps to the long arm of chromosome 7, 7q21.1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.