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Invitrogen
HHIP Polyclonal Antibody
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FIGURE: 1 / 13
HHIP Antibody (PA5-115355) in ICC/IF
Immunocytochemistry analysis of HHIP in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115355) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115355
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human HHIP(Accession Q96QV1), corresponding to amino acid residues E640-S690.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total Hhip.
Target Information
This gene encodes a protein similar to the mouse hedgehog-interacting protein, a regulatory component of the hedgehog signaling pathway. Members of the hedgehog family are evolutionarily conserved proteins which are involved in many fundamental processes in embryonic development, including anteroposterior patterns of limbs and regulation of left-right asymmetry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Hedgehog-interacting protein; Hedgehog-interacting protein-like; HHIP
Gene Aliases: HHIP; Hhip1; HIP; RGD1564108; UNQ5825/PRO19644
UniProt ID: (Human) Q96QV1, (Mouse) Q7TN16
Entrez Gene ID: (Human) 64399, (Rat) 291936, (Mouse) 15245
carbohydrate metabolic process
smoothened signaling pathway
neuroblast proliferation
dorsal/ventral pattern formation
negative regulation of signal transduction
regulation of fibroblast growth factor receptor signaling pathway
negative regulation of smoothened signaling pathway
skeletal system morphogenesis
oxidation-reduction process
epithelial tube branching involved in lung morphogenesis
signal transduction
organ morphogenesis
negative regulation of angiogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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