Hamburger Menu Button
Thermo Fisher Scientific Logo
Sign in
Don't have an account ? Create Account
  • Products
    • Antibodies
    • Cell Culture Media
    • Chemicals
    • Chromatography Columns and Cartridges
    • Lab Equipment
    • Lab Plasticware and Supplies
    • Microplates
    • Oligos, Primers, Probes and Genes
    • TaqMan Real-Time PCR Assays
    • See all product categories
  • Applications
    • Bioprocessing
    • Cell Culture and Transfection
    • Cell and Gene Therapy
    • Chromatography
    • Clinical Genomics
    • Digital Solutions
    • DNA and RNA Extraction and Analysis
    • Spectroscopy, Elemental and Isotope Analysis
    • See all applications and techniques
  • Services
    • 360° CDMO and CRO Solutions
    • CDMO Services
    • CRO Services
    • Custom Services
    • Financial and Leasing Services
    • Instrument Services
    • Lab Informatics
    • OEM and Commercial Supply
    • Training Services
    • Unity Lab Services
    • See all services
  • Help and Support
    • How to Order
    • Instrument Support
    • Learning Centers
    • Register for an Account
    • Technical Support Centers
    • See all help and support topics
  • Popular
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • Who We Serve
    • Biotech
    • Biopharma
    • CDMO
    • Lab Diagnostics
    • Industrial and Applied Sciences
  • Contact Us
  • Quick Order
  • Order Status and Tracking
  • Documents and Certificates
Thermo Fisher Scientific Logo

Search

Search All
Search button
          • Order Status
          • Quick Order
          • Sign in
            Sign in
            Don't have an account ? Create Account
            • Account
            • Check Order Status
            • Custom Products & Projects
            • Instrument Management
          • Primary Antibodies ›
          • HIF1A Antibodies

          Active Motif

          HIF-1 alpha Polyclonal Antibody

          2 References
          View all (69) HIF1A antibodies

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Tech Support
          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Tech Support
          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Tech Support

          Cite HIF-1 alpha Polyclonal Antibody

          Additional Information:
          {{banner.description}}
          {{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
          • Antibody Testing Data (3)
          HIF-1 alpha Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          HIF-1 alpha Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

          {{ $ctrl.currentElement.advancedVerification.fullName }}
          {{ $ctrl.currentElement.advancedVerification.text }}

          HIF-1 alpha Antibody (39665) in WB

          HIF-1 alpha antibody (pAb) tested by Western blot. Detection of HIF-1 alpha by Western blot analysis with 20 µg COS-7 nuclear extract either untreated (lane 1) or treated with CoCl2 (lane 2). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

          {{$ctrl.videoDesc}}

          {{$ctrl.videoLongDesc}}

          HIF-1 alpha Antibody in Western Blot (WB)
          HIF-1 alpha Antibody in ChIP Assay (ChIP)
          HIF-1 alpha Antibody in ChIP-Sequencing (ChIP-Seq)
          HIF-1 alpha Polyclonal Antibody

          Product Details

          39665

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          -

          ChIP assay (ChIP)

          5 µL
          -

          ChIP-sequencing (ChIP-Seq)

          5 µL
          -

          Miscellaneous PubMed (Misc)

          -
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          This HIF-1 alpha antibody was raised against a synthetic peptide corresponding to the C-terminal region of human HIF-1 alpha.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          Conc. Not Determined

          Storage buffer

          whole serum with 30% glycerol

          Contains

          0.035% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2614934

          Target Information

          HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: ARNT interacting protein; ARNT-interacting protein; ARNT2; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; hif 1; hif 1a; HIF-1-alpha; HIF-1alpha 786; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); Hypoxia-inducible factor 1-alpha; hypoxia-inducible factor1alpha; Member of PAS protein 1; member of PAS superfamily 1; PAS domain-containing protein 8; transcription factor; transcription factor; hypoxia-inducible factor 1a; unnamed protein product

          View more View less

          Gene Aliases: BHLHE78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; HIF1A; MOP1; PASD8

          View more View less

          UniProt ID: (Human) Q16665

          View more View less

          Entrez Gene ID: (Human) 3091

          View more View less

          Function(s)
          RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding bacterial-type RNA polymerase transcriptional repressor activity, sequence-specific DNA binding transcription corepressor binding transcription coactivator binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding p53 binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding ligand-dependent nuclear receptor binding enzyme binding protein kinase binding protein domain specific binding ubiquitin protein ligase binding histone deacetylase binding sequence-specific DNA binding protein heterodimerization activity protein dimerization activity Hsp90 protein binding RNA polymerase II sequence-specific DNA binding transcription factor binding E-box binding transcription regulator activator activity
          Process(es)
          response to reactive oxygen species angiogenesis blood vessel development response to hypoxia cellular glucose homeostasis neural crest cell migration epithelial to mesenchymal transition embryonic placenta development B-1 B cell homeostasis positive regulation of endothelial cell proliferation vasculature development heart looping positive regulation of neuroblast proliferation chondrocyte differentiation glandular epithelial cell differentiation glandular epithelial cell maturation connective tissue replacement involved in inflammatory response wound healing outflow tract morphogenesis cardiac ventricle morphogenesis lactate metabolic process regulation of glycolytic process regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter cellular iron ion homeostasis apoptotic process signal transduction neuroblast proliferation lactation visual learning response to iron ion regulation of gene expression vascular endothelial growth factor production positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression positive regulation of epithelial cell migration response to muscle activity positive regulation of macroautophagy axonal transport of mitochondrion neural fold elevation formation cerebral cortex development cell differentiation negative regulation of ossification bone mineralization negative regulation of bone mineralization positive regulation of vascular endothelial growth factor receptor signaling pathway TOR signaling negative regulation of TOR signaling oxygen homeostasis positive regulation of chemokine production regulation of transforming growth factor beta2 production collagen metabolic process cellular response to oxidative stress embryonic hemopoiesis insulin secretion involved in cellular response to glucose stimulus positive regulation of insulin secretion involved in cellular response to glucose stimulus regulation of cell proliferation hemoglobin biosynthetic process glucose homeostasis negative regulation of apoptotic process negative regulation of neuron apoptotic process positive regulation of blood vessel endothelial cell migration positive regulation of erythrocyte differentiation positive regulation of angiogenesis positive regulation of glycolytic process positive regulation of transcription, DNA-templated negative regulation of growth positive regulation of transcription from RNA polymerase II promoter muscle cell cellular homeostasis positive regulation of hormone biosynthetic process blood vessel morphogenesis digestive tract morphogenesis camera-type eye morphogenesis cartilage development neuron apoptotic process elastin metabolic process intestinal epithelial cell maturation epithelial cell differentiation involved in mammary gland alveolus development iris morphogenesis retina vasculature development in camera-type eye positive regulation of chemokine-mediated signaling pathway regulation of thymocyte apoptotic process negative regulation of thymocyte apoptotic process cellular response to interleukin-1 cellular response to hypoxia dopaminergic neuron differentiation mesenchymal cell apoptotic process hypoxia-inducible factor-1alpha signaling pathway cellular response to virus positive regulation of cytokine production involved in inflammatory response negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway positive regulation of nitric oxide metabolic process regulation of protein neddylation negative regulation of mesenchymal cell apoptotic process
          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

          Contact tech support
          Ordering Plus Icon Minus Icon
          • Order Status
          • Order Help
          • Quick Order
          • Supply Center
          • eProcurement
          Support Plus Icon Minus Icon
          • Help and Support
          • Contact Us
          • Technical Support Centers
          • Documents and Certificates
          • Report a Site Issue
          Resources Plus Icon Minus Icon
          • Learning Centers
          • Promotions
          • Events and Webinars
          • Social Media
          About Thermo Fisher Plus Icon Minus Icon
          • About Us About Us
          • Careers Careers
          • Investors Investors
          • News News
          • Social Responsibility Social Responsibility
          • Trademarks
          • Policies and Notices
          Our Portfolio Plus Icon Minus Icon
          • Thermo Scientific
          • Applied Biosystems
          • Invitrogen
          • Gibco
          • Ion Torrent
          • Fisher Scientific
          • Unity Lab Services
          • Patheon
          • PPD
          • Terms & Conditions
          • Privacy Policy
          • Price & Freight Policy
          © 2006-2026 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
          Hong Kong flag icon
          Hong Kong

          Your items have has been added!


          Host server : magellan-search-green-7d94cb4b65-mrft7:80/100.66.76.145:80.
          git-commit: c9e08c96761173abe34e68f880379696776a4827
          git-url: https://github.com/thermofisher/magellan-search
          git-branch: release/2.44.1-2026.02.97.1.0