Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
HIF-1 beta Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Product Details
PA3-16508
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Fish,
Ferret,
Human,
Mouse,
Sheep,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Fusion protein to human HIF-1 beta containing amino acids 496-789.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In Western blot, background may increase with whole cell extract preparations.
Lower dilutions may be needed for whole cell extract preps.
Suggested positive control: MCF-7 human nuclear extracts, MCF-7 whole cell lysate, antigen standard for ARNT (transient overexpression lysate).
Target Information
HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Diseases associated with HIF-1 beta dysfunction include hypoxia and renal cell carcinoma.HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Diseases associated with HIF-1 beta dysfunction include hypoxia and renal cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ARNT; ARNT protein; Aryl hydrocarbon receptor nuclear translocator; Aryl hydrocarbon receptor nuclear translocator 1; bHLHe2; Class E basic helix-loop-helix protein 2; Dioxin receptor, nuclear translocator; hif 1; HIF-1 beta; HIF-1-beta; HIF1 beta; HIF1-beta; hypoxia-inducible factor 1 beta; hypoxia-inducible factor 1, beta subunit; Hypoxia-inducible factor 1-beta
Gene Aliases: ARNT; Arnt1; BHLHE2; D3Ertd557e; Drnt; ESTM42; HIF-1-beta; HIF-1beta; HIF1-beta; HIF1B; HIF1BETA; mKIAA4051; TANGO; W08714
UniProt ID: (Human) Q5QP39, (Bovine) Q9BE97, (Rat) P41739, (Mouse) P53762
Entrez Gene ID: (Human) 405, (Bovine) 281010, (Sheep) 443285, (Rat) 25242, (Mouse) 11863
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription coactivator activity
aryl hydrocarbon receptor activity
protein binding
transcription factor binding
aryl hydrocarbon receptor binding
enhancer binding
sequence-specific DNA binding
protein heterodimerization activity
protein homodimerization activity
sequence-specific double-stranded DNA binding
transcription factor activity, transcription factor binding
DNA binding
signal transducer activity
protein dimerization activity
basic helix-loop-helix transcription factor
response to hypoxia
embryonic placenta development
positive regulation of endothelial cell proliferation
transcription, DNA-templated
positive regulation of vascular endothelial growth factor production
cell differentiation
intracellular receptor signaling pathway
positive regulation of vascular endothelial growth factor receptor signaling pathway
positive regulation of protein sumoylation
mRNA transcription from RNA polymerase II promoter
regulation of transcription from RNA polymerase II promoter in response to oxidative stress
positive regulation of erythrocyte differentiation
positive regulation of glycolytic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of hormone biosynthetic process
regulation of transcription from RNA polymerase II promoter in response to hypoxia
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
signal transduction
response to toxic substance
regulation of nucleic acid-templated transcription
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support