|Tested species reactivity||Bovine, Ferret, Human, Mouse, Sheep, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Fusion protein to HIF-1 beta containing residues 496-789.|
|Storage buffer||whole serum|
|Contains||0.02% sodium azide|
|Storage Conditions||-20°C or -80°C if preferred|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1:10-1:500|
|Gel Shift (GS)||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:150|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
In Western blot, background may increase with whole cell extract preparations.
Lower dilutions may be needed for whole cell extract preps.
Suggested positive control: MCF-7 human nuclear extracts, MCF-7 whole cell lysate, antigen standard for ARNT (transient overexpression lysate).
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A 5'-region polymorphism modulates promoter activity of the tumor suppressor gene MFSD2A.
PA3-16508 was used in immunoprecipitation to study the polymorphisms of MFSD2A gene and their effects on its promoter activity
|Colombo F,Falvella FS,Galvan A,Frullanti E,Kunitoh H,Ushijima T,Dragani TA||Molecular cancer (10:null)||2011|