Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
|Tested species reactivity||Mouse , Rat , Fish , Human , Primate , Sheep|
|Published species reactivity||Rat , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide derived from the C-terminus of mouse/human HIF-2 alpha protein.|
|Purification||Antigen affinity chromatography|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1:10-1:500|
|Flow Cytometry (Flow)||Assay-Dependent|
|Gel Shift (GS)||Assay-Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunoprecipitation (IP)||5 µg/1mg lysate|
|Western Blot (WB)||1 µg/ml-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
Suggested positive control: Cos7 CoCl2-treated nuclear extract, PC12 nuclear extracts.
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humans and endothelial cells of the embryonic and adult mouse, whereas HIF-1alpha functions primarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2 gene, which is known to be selectively expressed in endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Dose-dependent effects of allopurinol on human foreskin fibroblast cells and human umbilical vein endothelial cells under hypoxia.
PA1-16510 was used in western blot to study the effect of allopurinol treatment during hypoxia
|Sun Y,George J,Rocha S||PloS one (10:null)||2015|
HIF-mediated metabolic switching in bladder outlet obstruction mitigates the relaxing effect of mitochondrial inhibition.
PA1-16510 was used in western blot to study HIF-1alpha mediated metabolic switching and mitochondrial remodeling in bladder outlet obstruction
|Ekman M,Uvelius B,Albinsson S,Sw?rd K||Laboratory investigation; a journal of technical methods and pathology (94:557)||2014|
HIF2A, HLF, ECYT4, MOP2, PASD2, bHLHe73, HRF, Hif2a, HIF-2alpha
EPAS-1, HIF-2 alpha, HIF-2-alpha, HIF2 alpha, HIF2-alpha, endothelial PAS domain-containing protein 1, hypoxia inducible factor 2, alpha subunit, hypoxia inducible factor 2a, hypoxia-inducible factor 2 alpha, hypoxia-inducible factor 2-alpha, HIF-1-alpha-like factor, HIF-related factor, HIF1 alpha-like factor, HIF1alpha-like factor, HLF (HIF1alpha-like factor), Hif like protein, hypoxia inducible transcription factor 2alpha, mHLF, HIF-1alpha-like factor, PAS domain-containing protein 2, basic-helix-loop-helix-PAS protein MOP2, class E basic helix-loop-helix protein 73, member of PAS protein 2, Hypoxia inducible Factor 2 alpha, MOP2, Endothelial pas domain protein 1, Member of pas superfamily 2