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|Tested species reactivity||Fish, Hamster, Human, Mouse, Non-human primate, Sheep, Rat|
|Published species reactivity||Rat, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide derived from the C-terminus of mouse/human HIF-2 alpha protein.|
|Purification||Antigen affinity chromatography|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1:10-1:500|
|Flow Cytometry (Flow)||Assay-Dependent|
|Gel Shift (GS)||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunoprecipitation (IP)||5 µg/1mg lysate|
|Western Blot (WB)||1 µg/ml-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 4 publications below|
Suggested positive control: Cos7 CoCl2-treated nuclear extract, PC12 nuclear extracts.
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humans and endothelial cells of the embryonic and adult mouse, whereas HIF-1alpha functions primarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2 gene, which is known to be selectively expressed in endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cezanne regulates E2F1-dependent HIF2α expression.
PA1-16510 was used in western blot to report that expression of HIF2alpha is regulated by the deubiquitylase Cezanne in an E2F1-dependent manner.
|Moniz S,Bandarra D,Biddlestone J,Campbell KJ,Komander D,Bremm A,Rocha S||Journal of cell science (128:3082)||2015|
Dose-dependent effects of allopurinol on human foreskin fibroblast cells and human umbilical vein endothelial cells under hypoxia.
PA1-16510 was used in western blot to study the effect of allopurinol treatment during hypoxia
|Sun Y,George J,Rocha S||PloS one (10:null)||2015|
PITX1, a specificity determinant in the HIF-1α-mediated transcriptional response to hypoxia.
PA1-16510 was used in western blot to identify PITX1 as a key specificity factor for HIF-1alpha dependent gene expression.
|Mudie S,Bandarra D,Batie M,Biddlestone J,Moniz S,Ortmann B,Shmakova A,Rocha S||Cell cycle (Georgetown, Tex.) (13:3878)||2015|
HIF-mediated metabolic switching in bladder outlet obstruction mitigates the relaxing effect of mitochondrial inhibition.
PA1-16510 was used in western blot to study HIF-1alpha mediated metabolic switching and mitochondrial remodeling in bladder outlet obstruction
|Ekman M,Uvelius B,Albinsson S,Swärd K||Laboratory investigation; a journal of technical methods and pathology (94:557)||2014|
Basic-helix-loop-helix-PAS protein MOP2; bHLHe73; Class E basic helix-loop-helix protein 73; Endothelial pas domain protein 1; Endothelial PAS domain-containing protein 1; EPAS-1; Hif like protein; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF-2 alpha; HIF-2-alpha; HIF-related factor; HIF1 alpha-like factor; HIF1alpha-like factor; HIF2 alpha; HIF2-alpha; HLF; HLF (HIF1alpha-like factor); HRF; Hypoxia inducible Factor 2 alpha; hypoxia inducible factor 2, alpha subunit; hypoxia inducible factor 2a; hypoxia inducible transcription factor 2alpha; hypoxia-inducible factor 2 alpha; Hypoxia-inducible factor 2-alpha; Member of PAS protein 2; Member of pas superfamily 2; mHLF; MOP2; PAS domain-containing protein 2
BHLHE73; ECYT4; EPAS1; HIF-2alpha; HIF2A; HLF; HRF; MOP2; PASD2