Invitrogen

HIF-2 alpha Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

17 Published Figures

15 References

View all (31) HIF-2 alpha antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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HIF-2 alpha Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of HIF-2 alpha was performed using HepG2 cells treated with CoCl2 (300uM for 16hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HIF-2 alpha Rabbit Polyclonal Antibody (Product # PA1-16510) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the composite image showing more nuclear and less cytoplasmic localization in Hep G2 cells on treatment with CoCl2. Panel e represents untreated Hep G2 cells showing faint cytoplasmic localization. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

HIF-2 alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of HIF-2 alpha in formalin-fixed paraffin-embedded tissue sections of human placenta were probed for HIF-2 alpha/EPAS1 mRNA (ACD RNAScope Probe; Fast Red chromogen, ACD). Samples were incubated in HIF-2 alpha polyclonal antibody (Product # PA1-16510) using a dilution of 1:100 dilution with one-hour incubation at room temperature followed by anti-rabbit IgG VisUCyte HRP Polymer Antibody and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to trophoblastic cells.

HIF-2 alpha Antibody in Western Blot (WB) — Western blot was performed using anti-HIF2A-Polyclonal Antibody (Product # PA1-16510) a 120 kDa band was observed corresponding to HIF2A was observed across the cell lines tested and increased upon Cobalt Chloride treatment in Hep G2. Whole cell Extracts of (30ug lysate) of Hep G2 (Lane 1), Hep G2 treated with Cobalt Chloride (300uM for 16hrs) (Lane 2), IMR-32 (Lane 3), tissue extracts of Rat Testis (Lane 4) and Mouse Testis (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

HIF-2 alpha Antibody — Detection of altered subcellular localization of the target protein by cell treatment demonstrates antibody specificity. Immunofluorescence analysis using HIF-2 alpha Polyclonal Antibody (Product # PA1-16510), shows nuclear translocation of HIF-2 alpha in MCF7 cells upon cobalt chloride treatment. {TM}

Product Details

PA1-16510

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL	View 10 publications 10 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100	View 2 publications 2 publications
Immunohistochemistry (Frozen) (IHC (F))	1:100	-
Immunocytochemistry (ICC/IF)	1:100	-
Flow Cytometry (Flow)	Assay-Dependent	-
ELISA (ELISA)	1:100-1:2,000	-
Immunoprecipitation (IP)	5 µg/1 mg lysate	-
ChIP assay (ChIP)	1:10-1:500	-
Gel Shift (GS)	Assay-Dependent	-
In vitro Assay (IV)	Assay-Dependent	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Fish, Hamster, Human, Mouse, Non-human primate, Sheep, Rabbit, Reptile, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A peptide derived from the C-terminus of mouse/human HIF-2 alpha protein. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS
Contains	0.05% sodium azide
Storage conditions	-20°C
RRID	AB_2098236

Product Specific Information

Suggested positive control: Cos7 CoCl2-treated nuclear extract, PC12 nuclear extracts.

Target Information

HIF-2 alpha (Epas1) is a transcription factor involved in the induction of genes regulated by oxygen. HIF-2 alpha contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. It also regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of the lungs. Mutations in this gene are associated with erythrocytosis familial type 4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pharmacologic Inhibition of HIF-1α Attenuates Radiation-Induced Pulmonary Fibrosis in a Preclinical Image Guided Radiation Therapy.

International journal of radiation oncology, biology, physics

Nam JK,Kim AR,Choi SH,Kim JH,Han SC,Park S,Lee YJ,Kim J,Cho J,Lee HJ,Lee YJ

PA1-16510 was used in Immunohistochemistry (Paraffin) to examine the direct contribution of endothelial HIF-1α (EC-HIF1α) on radiation-induced pulmonary fibrosis.

Mon Feb 01 00:00:00 EST 2021

Myeloid Cell Hypoxia-Inducible Factors Promote Resolution of Inflammation in Experimental Colitis.

Frontiers in immunology

Lin N,Shay JES,Xie H,Lee DSM,Skuli N,Tang Q,Zhou Z,Azzam A,Meng H,Wang H,FitzGerald GA,Simon MC

Published figure using HIF-2 alpha polyclonal antibody (Product # PA1-16510) in Western Blot

Thu Sep 26 00:00:00 EDT 2019

Evaluation of a locked nucleic acid form of antisense oligo targeting HIF-1α in advanced hepatocellular carcinoma.

World journal of clinical oncology

Wu J,Contratto M,Shanbhogue KP,Manji GA,O'Neil BH,Noonan A,Tudor R,Lee R

PA1-16510 was used in Immunohistochemistry to indicate that RO7070179 might benefit HCC patients, and an early signal for clinical benefit can potentially be predicted through changes in either mRNA level or DCE-MRI within 1 cycle of therapy.

Sun Mar 24 00:00:00 EDT 2019

Serum and Tissue HIF-2 Alpha Expression in CIN, N-Acetyl Cysteine, and Sildenafil-Treated Rat Models: An Experimental Study.

Medicina (Kaunas, Lithuania)

Altintop I,Tatli M,Karakukcu C,Soyer Sarica Z,Hanım Yay A,Balcioglu E,Ozturk A

Published figure using HIF-2 alpha polyclonal antibody (Product # PA1-16510) in Immunohistochemistry (Paraffin)

Mon Jul 30 00:00:00 EDT 2018

SINHCAF/FAM60A and SIN3A specifically repress HIF-2α expression.

The Biochemical journal

Biddlestone J,Batie M,Bandarra D,Munoz I,Rocha S

Published figure using HIF-2 alpha polyclonal antibody (Product # PA1-16510) in Western Blot

Fri Jun 29 00:00:00 EDT 2018

Tumor-initiating cells of breast and prostate origin show alterations in the expression of genes related to iron metabolism.

Oncotarget

Rychtarcikova Z,Lettlova S,Tomkova V,Korenkova V,Langerova L,Simonova E,Zjablovskaja P,Alberich-Jorda M,Neuzil J,Truksa J

PA116510 was used in western blot to find the specific gene signature related to iron metabolism consisting of genes regulating iron uptake, mitochondrial FeS cluster biogenesis and hypoxic response

Tue Jan 24 00:00:00 EST 2017

Dose-dependent effects of allopurinol on human foreskin fibroblast cells and human umbilical vein endothelial cells under hypoxia.

PloS one

Sun Y,George J,Rocha S

Published figure using HIF-2 alpha polyclonal antibody (Product # PA1-16510) in Western Blot

Thu Feb 04 00:00:00 EST 2016

Epigenetic re-expression of HIF-2α suppresses soft tissue sarcoma growth.

Nature communications

Nakazawa MS,Eisinger-Mathason TS,Sadri N,Ochocki JD,Gade TP,Amin RK,Simon MC

Published figure using HIF-2 alpha polyclonal antibody (Product # PA1-16510) in Western Blot

Wed Feb 03 00:00:00 EST 2016

Epigenetic re-expression of HIF-2α suppresses soft tissue sarcoma growth.

Nature communications

Nakazawa MS,Eisinger-Mathason TS,Sadri N,Ochocki JD,Gade TP,Amin RK,Simon MC

Published figure using HIF-2 alpha polyclonal antibody (Product # PA1-16510) in Western Blot

Wed Feb 03 00:00:00 EST 2016

CDK-dependent phosphorylation of PHD1 on serine 130 alters its substrate preference in cells.

Journal of cell science

Ortmann B,Bensaddek D,Carvalhal S,Moser SC,Mudie S,Griffis ER,Swedlow JR,Lamond AI,Rocha S

PA1-16510 was used in western blot to investigate factors that control PHD1 activity

Fri Jan 01 00:00:00 EST 2016

PITX1, a specificity determinant in the HIF-1α-mediated transcriptional response to hypoxia.

Cell cycle (Georgetown, Tex.)

Mudie S,Bandarra D,Batie M,Biddlestone J,Moniz S,Ortmann B,Shmakova A,Rocha S

PA1-16510 was used in western blot to identify PITX1 as a key specificity factor for HIF-1alpha dependent gene expression.

Fri Sep 04 00:00:00 EDT 2015

Cezanne regulates E2F1-dependent HIF2α expression.

Journal of cell science

Moniz S,Bandarra D,Biddlestone J,Campbell KJ,Komander D,Bremm A,Rocha S

Published figure using HIF-2 alpha polyclonal antibody (Product # PA1-16510) in Western Blot

Sat Aug 15 00:00:00 EDT 2015

Systemic and Cerebral Vascular Endothelial Growth Factor Levels Increase in Murine Cerebral Malaria along with Increased Calpain and Caspase Activity and Can be Reduced by Erythropoietin Treatment.

Frontiers in immunology

Hempel C,Hoyer N,Kildemoes A,Jendresen CB,Kurtzhals JA

PA1-16510 was used in western blot to study vascular endothelial growth factor signaling in mice infected with Plasmodium berghei ANKA

Fri Jul 04 00:00:00 EDT 2014

HIF-mediated metabolic switching in bladder outlet obstruction mitigates the relaxing effect of mitochondrial inhibition.

Laboratory investigation; a journal of technical methods and pathology

Ekman M,Uvelius B,Albinsson S,Swärd K

PA1-16510 was used in western blot to study HIF-1alpha mediated metabolic switching and mitochondrial remodeling in bladder outlet obstruction

Thu May 01 00:00:00 EDT 2014

The chromatin remodeler ISWI regulates the cellular response to hypoxia: role of FIH.

Molecular biology of the cell

Melvin A,Mudie S,Rocha S

Published figure using HIF-2 alpha polyclonal antibody (Product # PA1-16510) in Western Blot

Tue Nov 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: Basic-helix-loop-helix-PAS protein MOP2; bHLHe73; Class E basic helix-loop-helix protein 73; endothelial PAS domain protein 1; Endothelial PAS domain-containing protein 1; EPAS; EPAS-1; Hif like protein; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF-2 alpha; HIF-2-alpha; HIF-2alpha; HIF-related factor; HIF1 alpha-like factor; HIF1alpha-like factor; HIF2 alpha; HIF2-alpha; HLF; HLF (HIF1alpha-like factor); HRF; hypoxia inducible factor 2, alpha subunit; hypoxia inducible factor 2a; hypoxia inducible transcription factor 2alpha; hypoxia-inducible factor 2 alpha; Hypoxia-inducible factor 2-alpha; Member of PAS protein 2; mHLF; PAS domain-containing protein 2

Gene Aliases: BHLHE73; ECYT4; EPAS; EPAS1; HIF-2alpha; HIF2A; HLF; HRF; MOP2; PASD2

UniProt ID: (Human) Q99814, (Mouse) P97481, (Rat) Q9JHS1

Entrez Gene ID: (Rabbit) 100348809, (Sheep) 443286, (Human) 2034, (Mouse) 13819, (Chinese hamster) 100771160, (Rat) 29452

Molecular Function: basic helix-loop-helix transcription factor