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      HIF-2 alpha Polyclonal Antibody

      See 13 other HIF-2 alpha antibodies ›
      HIF-2 alpha Polyclonal Antibody for Western Blot, IF, ICC, IHC, IHC (P), Flow, IP, ChIP, ELISA and GS

      Catalog#: PA1-16510 Invitrogen

      Catalog # Size Price (USD) Qty Datasheet
      PA1-16510 100 µL
      375.00
      Please enter a quantity in the range of 1 to 199.
      Suggested Secondary Antibodies

      Anti-Rabbit Secondary Antibodies (264)
      Alexa Fluor® Anti-Rabbit Secondary Antibodies (69)
      HRP Anti-Rabbit Secondary Antibodies (30)

      Product Details

      References

      Bioinformatics

      Documents
      Details
      Tested species reactivity Fish, Hamster, Human, Mouse, Non-human primate, Sheep, Rat
      Published species reactivity Rat, Human, Not Applicable
      Host / Isotype Rabbit / IgG
      Class Polyclonal
      Type Antibody
      Immunogen A peptide derived from the C-terminus of mouse/human HIF-2 alpha protein.
      Conjugate Unconjugated
      Form Liquid
      Concentration 1 mg/ml
      Purification Antigen affinity chromatography
      Storage buffer PBS
      Contains 0.05% sodium azide
      Storage Conditions 4° C, do not freeze
      Tested Applications Dilution *
      ChIP assay (ChIP) 1:10-1:500
      ELISA (ELISA) 1:100-1:2000
      Flow Cytometry (Flow) Assay-Dependent
      Gel Shift (GS) Assay-Dependent
      Immunocytochemistry (ICC) 1:100
      Immunofluorescence (IF) 1:100
      Immunohistochemistry (IHC) 1:100
      Immunohistochemistry (Paraffin) (IHC (P)) 1:100
      Immunoprecipitation (IP) 5 µg/1mg lysate
      Western Blot (WB) 1 µg/ml-2 µg/ml

      * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.

      Published Applications
      Immunohistochemistry (IHC) See 1 publications below
      Miscellaneous PubMed (MISC) See 1 publications below
      Western Blot (WB) See 6 publications below

      Product Specific Information

      Suggested positive control: Cos7 CoCl2-treated nuclear extract, PC12 nuclear extracts.

      Background/Target Information

      Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humans and endothelial cells of the embryonic and adult mouse, whereas HIF-1alpha functions primarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2 gene, which is known to be selectively expressed in endothelial cells.

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      References

      Immunohistochemistry

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Not Applicable Not Cited Epigenetic re-expression of HIF-2¿ suppresses soft tissue sarcoma growth.
      PA1-16510 was used in immunohistochemistry to analyze the suppression of soft tissue sarcoma growth due to epigenetic re-expression of HIF-2 alpha       		Nakazawa MS,Eisinger-Mathason TS,Sadri N,Ochocki JD,Gade TP,Amin RK,Simon MC Nature communications (7:null) 2016

      Miscellaneous PubMed

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Human 1:1000 CDK-dependent phosphorylation of PHD1 on serine 130 alters its substrate preference in cells.
      PA1-16510 was used in western blot to investigate factors that control PHD1 activity       		Ortmann B,Bensaddek D,Carvalhal S,Moser SC,Mudie S,Griffis ER,Swedlow JR,Lamond AI,Rocha S Journal of cell science (129:191) 2016

      Western Blot

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Human Not Cited Cezanne regulates E2F1-dependent HIF2¿ expression.
      PA1-16510 was used in western blot to report that expression of HIF2alpha is regulated by the deubiquitylase Cezanne in an E2F1-dependent manner.       		Moniz S,Bandarra D,Biddlestone J,Campbell KJ,Komander D,Bremm A,Rocha S Journal of cell science (128:3082) 2015
      Human 1:1000 Dose-dependent effects of allopurinol on human foreskin fibroblast cells and human umbilical vein endothelial cells under hypoxia.
      PA1-16510 was used in western blot to study the effect of allopurinol treatment during hypoxia       		Sun Y,George J,Rocha S PloS one (10:null) 2015
      Human Not Cited PITX1, a specificity determinant in the HIF-1¿-mediated transcriptional response to hypoxia.
      PA1-16510 was used in western blot to identify PITX1 as a key specificity factor for HIF-1alpha dependent gene expression.       		Mudie S,Bandarra D,Batie M,Biddlestone J,Moniz S,Ortmann B,Shmakova A,Rocha S Cell cycle (Georgetown, Tex.) (13:3878) 2015
      Not Applicable 1:500 Systemic and Cerebral Vascular Endothelial Growth Factor Levels Increase in Murine Cerebral Malaria along with Increased Calpain and Caspase Activity and Can be Reduced by Erythropoietin Treatment.
      PA1-16510 was used in western blot to study vascular endothelial growth factor signaling in mice infected with Plasmodium berghei ANKA       		Hempel C,Hoyer N,Kildemoes A,Jendresen CB,Kurtzhals JA Frontiers in immunology (5:null) 2014
      Rat 1:200 HIF-mediated metabolic switching in bladder outlet obstruction mitigates the relaxing effect of mitochondrial inhibition.
      PA1-16510 was used in western blot to study HIF-1alpha mediated metabolic switching and mitochondrial remodeling in bladder outlet obstruction       		Ekman M,Uvelius B,Albinsson S,Swärd K Laboratory investigation; a journal of technical methods and pathology (94:557) 2014
      Not Applicable Not Cited The chromatin remodeler ISWI regulates the cellular response to hypoxia: role of FIH.
      PA1-16510 was used in western blot to study the role of chromatin remodeling in the hypoxia response       		Melvin A,Mudie S,Rocha S Molecular biology of the cell (22:4171) 2011

      Bioinformatics

      Protein Aliases:

      Basic-helix-loop-helix-PAS protein MOP2; bHLHe73; Class E basic helix-loop-helix protein 73; Endothelial pas domain protein 1; Endothelial PAS domain-containing protein 1; EPAS-1; Hif like protein; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF-2 alpha; HIF-2-alpha; HIF-related factor; HIF1 alpha-like factor; HIF1alpha-like factor; HIF2 alpha; HIF2-alpha; HLF; HLF (HIF1alpha-like factor); HRF; Hypoxia inducible Factor 2 alpha; hypoxia inducible factor 2, alpha subunit; hypoxia inducible factor 2a; hypoxia inducible transcription factor 2alpha; hypoxia-inducible factor 2 alpha; Hypoxia-inducible factor 2-alpha; Member of PAS protein 2; Member of pas superfamily 2; mHLF; MOP2; PAS domain-containing protein 2

      Gene Aliases:

      BHLHE73; ECYT4; EPAS1; HIF-2alpha; HIF2A; HLF; HRF; MOP2; PASD2

      UniProt ID:

      (Human) Q99814, (Rat) Q9JHS1, (Mouse) P97481

      Entrez Gene ID:

      (Human) 2034, (Rat) 29452, (Mouse) 13819

      Molecular Function:

      basic helix-loop-helix transcription factor nucleic acid binding transcription factor

      Function(s) Process(es)

      Documents

      Material safety data sheets (MSDS)

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