Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1-2 µg/mL | View 10 publications 10 publications |
Immunohistochemistry (IHC) |
- | View 2 publications 2 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:100 | View 2 publications 2 publications |
Immunohistochemistry (Frozen) (IHC (F)) |
1:100 | - |
Immunocytochemistry (ICC/IF) |
1:100 | - |
Flow Cytometry (Flow) |
Assay-Dependent | - |
ELISA (ELISA) |
1:100-1:2,000 | - |
Immunoprecipitation (IP) |
5 µg/1 mg lysate | - |
ChIP assay (ChIP) |
1:10-1:500 | - |
Gel Shift (GS) |
Assay-Dependent | - |
In vitro Assay (IV) |
Assay-Dependent | - |
Miscellaneous PubMed (Misc) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Fish, Hamster, Human, Mouse, Non-human primate, Sheep, Rabbit, Reptile, Rat |
Published species |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
A peptide derived from the C-terminus of mouse/human HIF-2 alpha protein. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS |
Contains |
0.05% sodium azide |
Storage conditions |
-20°C |
RRID |
AB_2098236 |
Suggested positive control: Cos7 CoCl2-treated nuclear extract, PC12 nuclear extracts.
HIF-2 alpha (Epas1) is a transcription factor involved in the induction of genes regulated by oxygen. HIF-2 alpha contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. It also regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of the lungs. Mutations in this gene are associated with erythrocytosis familial type 4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Basic-helix-loop-helix-PAS protein MOP2; bHLHe73; Class E basic helix-loop-helix protein 73; endothelial PAS domain protein 1; Endothelial PAS domain-containing protein 1; EPAS; EPAS-1; Hif like protein; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF-2 alpha; HIF-2-alpha; HIF-2alpha; HIF-related factor; HIF1 alpha-like factor; HIF1alpha-like factor; HIF2 alpha; HIF2-alpha; HLF; HLF (HIF1alpha-like factor); HRF; hypoxia inducible factor 2, alpha subunit; hypoxia inducible factor 2a; hypoxia inducible transcription factor 2alpha; hypoxia-inducible factor 2 alpha; Hypoxia-inducible factor 2-alpha; Member of PAS protein 2; mHLF; PAS domain-containing protein 2
Gene Aliases: BHLHE73; ECYT4; EPAS; EPAS1; HIF-2alpha; HIF2A; HLF; HRF; MOP2; PASD2
UniProt ID: (Human) Q99814, (Mouse) P97481, (Rat) Q9JHS1
Entrez Gene ID: (Rabbit) 100348809, (Sheep) 443286, (Human) 2034, (Mouse) 13819, (Chinese hamster) 100771160, (Rat) 29452
Molecular Function:
basic helix-loop-helix transcription factor
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