Invitrogen

HIF-2 alpha Polyclonal Antibody

HIF-2 alpha Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 33

HIF-2 alpha Antibody (PA1-16510) in ICC/IF

Immunofluorescence analysis of HIF-2 alpha was performed using HepG2 cells treated with CoCl2 (300uM for 16hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HIF-2 alpha Rabbit Polyclonal Antibody (Product # PA1-16510) at 1:100 dilution in 0.1% BSA and in... View More

HIF-2 alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HIF-2 alpha Antibody in Western Blot (WB)

HIF-2 alpha Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

PA1-16510

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

1:100

Immunocytochemistry (ICC/IF)

1:100

Flow Cytometry (Flow)

Assay-Dependent

ELISA (ELISA)

1:100-1:2,000

Immunoprecipitation (IP)

5 µg/1 mg lysate

ChIP assay (ChIP)

1:10-1:500

Gel Shift (GS)

Assay-Dependent

In vitro Assay (IV)

Assay-Dependent

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Fish, Hamster, Human, Mouse, Non-human primate, Sheep, Rabbit, Reptile, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A peptide derived from the C-terminus of mouse/human HIF-2 alpha protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2098236

Product Specific Information

Suggested positive control: Cos7 CoCl2-treated nuclear extract, PC12 nuclear extracts.

Target Information

HIF-2 alpha (Epas1) is a transcription factor involved in the induction of genes regulated by oxygen. HIF-2 alpha contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. It also regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of the lungs. Mutations in this gene are associated with erythrocytosis familial type 4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Antioxidants stimulate BACH1-dependent tumor angiogenesis.

The Journal of clinical investigation

Wang T,Dong Y,Huang Z,Zhang G,Zhao Y,Yao H,Hu J,Tüksammel E,Cai H,Liang N,Xu X,Yang X,Schmidt S,Qiao X,Schlisio S,Strömblad S,Qian H,Jiang C,Treuter E,Bergo MO

Published figure using HIF-2 alpha polyclonal antibody (Product # PA1-16510) in Western Blot

Mon Oct 16 00:00:00 UTC 2023

Antioxidants stimulate BACH1-dependent tumor angiogenesis.

The Journal of clinical investigation

Wang T,Dong Y,Huang Z,Zhang G,Zhao Y,Yao H,Hu J,Tüksammel E,Cai H,Liang N,Xu X,Yang X,Schmidt S,Qiao X,Schlisio S,Strömblad S,Qian H,Jiang C,Treuter E,Bergo MO

Published figure using HIF-2 alpha polyclonal antibody (Product # PA1-16510) in Western Blot

Mon Oct 16 00:00:00 UTC 2023

CRISPR screening identifies the deubiquitylase ATXN3 as a PD-L1-positive regulator for tumor immune evasion.

The Journal of clinical investigation

Wang S,Iyer R,Han X,Wei J,Li N,Cheng Y,Zhou Y,Gao Q,Zhang L,Yan M,Sun Z,Fang D

PA1-16510 was used in Immunohistochemistry to identify what we believe to be a previously unknown deubiquitinase, ATXN3, as a positive regulator for PD-L1 transcription and provide a rationale for targeting ATXN3 to sensitize checkpoint blockade antitumor immunotherapy.

Fri Dec 01 00:00:00 UTC 2023

CRISPR screening identifies the deubiquitylase ATXN3 as a PD-L1-positive regulator for tumor immune evasion.

The Journal of clinical investigation

Wang S,Iyer R,Han X,Wei J,Li N,Cheng Y,Zhou Y,Gao Q,Zhang L,Yan M,Sun Z,Fang D

Fri Dec 01 00:00:00 UTC 2023

Molecular and Functional Characteristics of Airway Epithelium under Chronic Hypoxia.

International journal of molecular sciences

Wong SL,Kardia E,Vijayan A,Umashankar B,Pandzic E,Zhong L,Jaffe A,Waters SA

Published figure using HIF-2 alpha polyclonal antibody (Product # PA1-16510) in Western Blot

Thu Mar 30 00:00:00 UTC 2023

Pharmacologic Inhibition of HIF-1α Attenuates Radiation-Induced Pulmonary Fibrosis in a Preclinical Image Guided Radiation Therapy.

International journal of radiation oncology, biology, physics

Nam JK,Kim AR,Choi SH,Kim JH,Han SC,Park S,Lee YJ,Kim J,Cho J,Lee HJ,Lee YJ

PA1-16510 was used in Immunohistochemistry (Paraffin) to examine the direct contribution of endothelial HIF-1α (EC-HIF1α) on radiation-induced pulmonary fibrosis.

Mon Feb 01 00:00:00 UTC 2021

Evaluation of a locked nucleic acid form of antisense oligo targeting HIF-1α in advanced hepatocellular carcinoma.

World journal of clinical oncology

Wu J,Contratto M,Shanbhogue KP,Manji GA,O'Neil BH,Noonan A,Tudor R,Lee R

PA1-16510 was used in Immunohistochemistry to indicate that RO7070179 might benefit HCC patients, and an early signal for clinical benefit can potentially be predicted through changes in either mRNA level or DCE-MRI within 1 cycle of therapy.

Sun Mar 24 00:00:00 UTC 2019

SINHCAF/FAM60A and SIN3A specifically repress HIF-2α expression.

The Biochemical journal

Biddlestone J,Batie M,Bandarra D,Munoz I,Rocha S

Published figure using HIF-2 alpha polyclonal antibody (Product # PA1-16510) in Western Blot

Fri Jun 29 00:00:00 UTC 2018

Serum and Tissue HIF-2 Alpha Expression in CIN, N-Acetyl Cysteine, and Sildenafil-Treated Rat Models: An Experimental Study.

Medicina (Kaunas, Lithuania)

Altintop I,Tatli M,Karakukcu C,Soyer Sarica Z,Hanım Yay A,Balcioglu E,Ozturk A

Published figure using HIF-2 alpha polyclonal antibody (Product # PA1-16510) in Immunohistochemistry (Paraffin)

Mon Jul 30 00:00:00 UTC 2018

Myeloid Cell Hypoxia-Inducible Factors Promote Resolution of Inflammation in Experimental Colitis.

Frontiers in immunology

Lin N,Shay JES,Xie H,Lee DSM,Skuli N,Tang Q,Zhou Z,Azzam A,Meng H,Wang H,FitzGerald GA,Simon MC

Published figure using HIF-2 alpha polyclonal antibody (Product # PA1-16510) in Western Blot

Thu Sep 26 00:00:00 UTC 2019

Tumor-initiating cells of breast and prostate origin show alterations in the expression of genes related to iron metabolism.

Oncotarget

Rychtarcikova Z,Lettlova S,Tomkova V,Korenkova V,Langerova L,Simonova E,Zjablovskaja P,Alberich-Jorda M,Neuzil J,Truksa J

PA116510 was used in western blot to find the specific gene signature related to iron metabolism consisting of genes regulating iron uptake, mitochondrial FeS cluster biogenesis and hypoxic response

Tue Jan 24 00:00:00 UTC 2017

CDK-dependent phosphorylation of PHD1 on serine 130 alters its substrate preference in cells.

Journal of cell science

Ortmann B,Bensaddek D,Carvalhal S,Moser SC,Mudie S,Griffis ER,Swedlow JR,Lamond AI,Rocha S

PA1-16510 was used in western blot to investigate factors that control PHD1 activity

Fri Jan 01 00:00:00 UTC 2016

Epigenetic re-expression of HIF-2α suppresses soft tissue sarcoma growth.

Nature communications

Nakazawa MS,Eisinger-Mathason TS,Sadri N,Ochocki JD,Gade TP,Amin RK,Simon MC

PA1-16510 was used in immunohistochemistry to analyze the suppression of soft tissue sarcoma growth due to epigenetic re-expression of HIF-2 alpha

Wed Feb 03 00:00:00 UTC 2016

Epigenetic re-expression of HIF-2α suppresses soft tissue sarcoma growth.

Nature communications

Nakazawa MS,Eisinger-Mathason TS,Sadri N,Ochocki JD,Gade TP,Amin RK,Simon MC

PA1-16510 was used in immunohistochemistry to analyze the suppression of soft tissue sarcoma growth due to epigenetic re-expression of HIF-2 alpha

Wed Feb 03 00:00:00 UTC 2016

Cezanne regulates E2F1-dependent HIF2α expression.

Journal of cell science

Moniz S,Bandarra D,Biddlestone J,Campbell KJ,Komander D,Bremm A,Rocha S

PA1-16510 was used in western blot to report that expression of HIF2alpha is regulated by the deubiquitylase Cezanne in an E2F1-dependent manner.

Sat Aug 15 00:00:00 UTC 2015

Dose-dependent effects of allopurinol on human foreskin fibroblast cells and human umbilical vein endothelial cells under hypoxia.

PloS one

Sun Y,George J,Rocha S

PA1-16510 was used in western blot to study the effect of allopurinol treatment during hypoxia

Thu Feb 04 00:00:00 UTC 2016

Systemic and Cerebral Vascular Endothelial Growth Factor Levels Increase in Murine Cerebral Malaria along with Increased Calpain and Caspase Activity and Can be Reduced by Erythropoietin Treatment.

Frontiers in immunology

Hempel C,Hoyer N,Kildemoes A,Jendresen CB,Kurtzhals JA

PA1-16510 was used in western blot to study vascular endothelial growth factor signaling in mice infected with Plasmodium berghei ANKA

Fri Jul 04 00:00:00 UTC 2014

HIF-mediated metabolic switching in bladder outlet obstruction mitigates the relaxing effect of mitochondrial inhibition.

Laboratory investigation; a journal of technical methods and pathology

Ekman M,Uvelius B,Albinsson S,Swärd K

PA1-16510 was used in western blot to study HIF-1alpha mediated metabolic switching and mitochondrial remodeling in bladder outlet obstruction

Thu May 01 00:00:00 UTC 2014

PITX1, a specificity determinant in the HIF-1α-mediated transcriptional response to hypoxia.

Cell cycle (Georgetown, Tex.)

Mudie S,Bandarra D,Batie M,Biddlestone J,Moniz S,Ortmann B,Shmakova A,Rocha S

PA1-16510 was used in western blot to identify PITX1 as a key specificity factor for HIF-1alpha dependent gene expression.

Fri Sep 04 00:00:00 UTC 2015

The chromatin remodeler ISWI regulates the cellular response to hypoxia: role of FIH.

Molecular biology of the cell

Melvin A,Mudie S,Rocha S

PA1-16510 was used in western blot to study the role of chromatin remodeling in the hypoxia response

Tue Nov 01 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: Basic-helix-loop-helix-PAS protein MOP2; bHLHe73; Class E basic helix-loop-helix protein 73; endothelial PAS domain protein 1; Endothelial PAS domain-containing protein 1; EPAS; EPAS-1; Hif like protein; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF-2 alpha; HIF-2-alpha; HIF-2alpha; HIF-related factor; HIF1 alpha-like factor; HIF1alpha-like factor; HIF2 alpha; HIF2-alpha; HLF; HLF (HIF1alpha-like factor); HRF; hypoxia inducible factor 2, alpha subunit; hypoxia inducible factor 2a; hypoxia inducible transcription factor 2alpha; hypoxia-inducible factor 2 alpha; Hypoxia-inducible factor 2-alpha; Member of PAS protein 2; mHLF; PAS domain-containing protein 2

View more View less

Gene Aliases: BHLHE73; ECYT4; EPAS; EPAS1; HIF-2alpha; HIF2A; HLF; HRF; MOP2; PASD2

View more View less

UniProt ID: (Human) Q99814, (Mouse) P97481, (Rat) Q9JHS1

View more View less

Entrez Gene ID: (Rabbit) 100348809, (Sheep) 443286, (Human) 2034, (Mouse) 13819, (Chinese hamster) 100771160, (Rat) 29452

View more View less

Function(s)

RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding protein binding transcription factor binding histone acetyltransferase binding sequence-specific DNA binding protein heterodimerization activity transcription factor activity, sequence-specific DNA binding signal transducer activity protein dimerization activity basic helix-loop-helix transcription factor

Process(es)

angiogenesis response to hypoxia embryonic placenta development blood vessel remodeling regulation of heart rate transcription from RNA polymerase II promoter mitochondrion organization signal transduction visual perception erythrocyte differentiation lung development somatic stem cell population maintenance norepinephrine metabolic process surfactant homeostasis regulation of transcription from RNA polymerase II promoter in response to oxidative stress positive regulation of transcription from RNA polymerase II promoter cell maturation myoblast fate commitment iron ion homeostasis regulation of transcription from RNA polymerase II promoter in response to hypoxia cellular response to hypoxia transcription, DNA-templated regulation of transcription, DNA-templated response to oxidative stress multicellular organism development hemopoiesis cell differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

HIF-2 alpha Polyclonal Antibody

HIF-2 alpha Antibody (PA1-16510) in ICC/IF

Product Details

PA1-16510

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Gel Shift (GS)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!