|ChIP assay (ChIP)||1:10-1:500|
|Flow Cytometry (Flow)||Assay-Dependent|
|Gel Shift (GS)||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunoprecipitation (IP)||5 µg/1mg lysate|
|Western Blot (WB)||1 µg/ml-2 µg/ml|
|Immunohistochemistry (IHC)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Western Blot (WB)||See 6 publications below|
|Tested Species reactivity||Fish, Hamster, Human, Mouse, Non-human primate, Sheep, Rat|
|Published species reactivity||Rat , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide derived from the C-terminus of mouse/human HIF-2 alpha protein.|
|Purification||Antigen affinity chromatography|
|Contains||0.05% sodium azide|
|Storage conditions||4° C, do not freeze|
Suggested positive control: Cos7 CoCl2-treated nuclear extract, PC12 nuclear extracts.
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humans and endothelial cells of the embryonic and adult mouse, whereas HIF-1alpha functions primarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2 gene, which is known to be selectively expressed in endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Basic-helix-loop-helix-PAS protein MOP2; bHLHe73; Class E basic helix-loop-helix protein 73; Endothelial pas domain protein 1; Endothelial PAS domain-containing protein 1; EPAS-1; Hif like protein; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF-2 alpha; HIF-2-alpha; HIF-related factor; HIF1 alpha-like factor; HIF1alpha-like factor; HIF2 alpha; HIF2-alpha; HLF; HLF (HIF1alpha-like factor); HRF; Hypoxia inducible Factor 2 alpha; hypoxia inducible factor 2, alpha subunit; hypoxia inducible factor 2a; hypoxia inducible transcription factor 2alpha; hypoxia-inducible factor 2 alpha; Hypoxia-inducible factor 2-alpha; Member of PAS protein 2; Member of pas superfamily 2; mHLF; MOP2; PAS domain-containing protein 2
Gene Aliases: BHLHE73; ECYT4; EPAS1; HIF-2alpha; HIF2A; HLF; HRF; MOP2; PASD2
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