Invitrogen

HIF-2 alpha Polyclonal Antibody

Product Image Gallery

HIF-2 alpha Antibody — Figure 1 Arnt deletion in macrophages disrupts both HIF-1alpha and HIF -2alpha transcriptional activity. (A) PCR analysis of genomic DNA prepared from bone marrow-derived macrophages (BMDMs) and tail of LysMCre and LysMCre;Arnt fl / fl mice. The 2 lox band is the floxed allele of Arnt , and the 1 lox band indicates successful Cre-mediated recombination. (B) Western blotting of murine ARNT, HIF-1alpha, and HIF-2alpha in protein lysates prepared from LysMCre and LysMCre;Arnt fl / fl BMDMs cultured under 21% O 2 (normoxia) or 0.5% O 2 (hypoxia) for 24 h. Beta-actin was used as loading control. Densitometry was applied for quantification of HIF-1alpha and HIF-2alpha normalized to beta-actin. The numbers indicate average values of duplicated samples. (C-E) Real-time quantitative PCR (RT-qPCR) analysis of HIF target genes in LysMCre ( n = 3; mean +- s.e.m.) and LysMCre;Arnt fl / fl ( n = 3; mean +- s.e.m.) BMDMs cultured under 21% O 2 or 0.5% O 2 for 24 h. Two-way ANOVA, * p < 0.05, ** p < 0.01, and **** p < 0.0001.

HIF-2 alpha Antibody in Immunohistochemistry (IHC) — Hif-2 alpha immunoreactivity in human cardiac myocytes stained with PA1-16510.

HIF-2 alpha Antibody in Western Blot (WB) — Western Blot analysis on normoxic and hypoxic nuclear rat cell lysates using Product # PA1-16510, anti-HIF-2 alpha.

HIF-2 alpha Antibody in Immunocytochemistry (ICC) — Detection of HIF-2 Alpha (Green) in RCC4 cells using Product # PA1-16510. Nuclei (Blue) are counterstained with Hoechst 33258.

Product Details

Tested Applications

Dilution

ChIP assay (ChIP)

1:10-1:500

ELISA (ELISA)

1:100-1:2000

Flow Cytometry (Flow)

Assay-Dependent

Gel Shift (GS)

Assay-Dependent

Immunocytochemistry (ICC)

1:100

Immunofluorescence (IF)

1:100

Immunohistochemistry (Frozen) (IHC (F))

1:100

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunoprecipitation (IP)

5 µg/1 mg lysate

Western Blot (WB)

1-2 µg/mL

Published Applications

Western Blot (WB)

See 10 publications below

Immunohistochemistry (IHC)

See 2 publications below

Immunohistochemistry (Paraffin) (IHC (P))

See 1 publication below

Miscellaneous PubMed (MISC)

See 1 publication below

Product Specifications

Species Reactivity

Fish, Hamster, Human, Mouse, Non-human primate, Sheep, Rat

Published species

Human, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A peptide derived from the C-terminus of mouse/human HIF-2 alpha protein.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20°C

RRID

AB_2098236

Product Specific Information

Suggested positive control: Cos7 CoCl2-treated nuclear extract, PC12 nuclear extracts.

Target Information

HIF-2 alpha (Epas1) is a transcription factor involved in the induction of genes regulated by oxygen. HIF-2 alpha contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. It also regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of the lungs. Mutations in this gene are associated with erythrocytosis familial type 4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: basic-helix-loop-helix-PAS protein MOP2; class E basic helix-loop-helix protein 73; Endothelial pas domain protein 1; Endothelial PAS domain-containing protein 1; EPAS; EPAS-1; Hif like protein; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF-2 alpha; HIF-2-alpha; HIF-2alpha; HIF-related factor; HIF1 alpha-like factor; HIF1alpha-like factor; HIF2 alpha; HIF2-alpha; HLF (HIF1alpha-like factor); Hypoxia inducible Factor 2 alpha; hypoxia inducible factor 2, alpha subunit; hypoxia inducible factor 2a; hypoxia inducible transcription factor 2alpha; hypoxia-inducible factor 2 alpha; hypoxia-inducible factor 2-alpha; member of PAS protein 2; Member of pas superfamily 2; mHLF; MOP2; PAS domain-containing protein 2

Gene Aliases: bHLHe73; ECYT4; EPAS; HIF-2alpha; HIF2A; HLF; HRF; MOP2; PASD2

Entrez Gene ID: (Sheep) 443286, (Human) 2034, (Mouse) 13819, (Chinese hamster) 100771160, (Rat) 29452

Molecular Function: basic helix-loop-helix transcription factor nucleic acid binding transcription factor