|Tested species reactivity||Fish, Hamster, Human, Mouse, Non-human primate, Sheep, Rat|
|Published species reactivity||Rat, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide derived from the C-terminus of mouse/human HIF-2 alpha protein.|
|Purification||Antigen affinity chromatography|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1:10-1:500|
|Flow Cytometry (Flow)||Assay-Dependent|
|Gel Shift (GS)||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunoprecipitation (IP)||5 µg/1mg lysate|
|Western Blot (WB)||1 µg/ml-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: Cos7 CoCl2-treated nuclear extract, PC12 nuclear extracts.
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humans and endothelial cells of the embryonic and adult mouse, whereas HIF-1alpha functions primarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2 gene, which is known to be selectively expressed in endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Epigenetic re-expression of HIF-2¿ suppresses soft tissue sarcoma growth.
PA1-16510 was used in immunohistochemistry to analyze the suppression of soft tissue sarcoma growth due to epigenetic re-expression of HIF-2 alpha
|Nakazawa MS,Eisinger-Mathason TS,Sadri N,Ochocki JD,Gade TP,Amin RK,Simon MC||Nature communications (7:null)||2016|
CDK-dependent phosphorylation of PHD1 on serine 130 alters its substrate preference in cells.
PA1-16510 was used in western blot to investigate factors that control PHD1 activity
|Ortmann B,Bensaddek D,Carvalhal S,Moser SC,Mudie S,Griffis ER,Swedlow JR,Lamond AI,Rocha S||Journal of cell science (129:191)||2016|
Cezanne regulates E2F1-dependent HIF2¿ expression.
PA1-16510 was used in western blot to report that expression of HIF2alpha is regulated by the deubiquitylase Cezanne in an E2F1-dependent manner.
|Moniz S,Bandarra D,Biddlestone J,Campbell KJ,Komander D,Bremm A,Rocha S||Journal of cell science (128:3082)||2015|
Dose-dependent effects of allopurinol on human foreskin fibroblast cells and human umbilical vein endothelial cells under hypoxia.
PA1-16510 was used in western blot to study the effect of allopurinol treatment during hypoxia
|Sun Y,George J,Rocha S||PloS one (10:null)||2015|
PITX1, a specificity determinant in the HIF-1¿-mediated transcriptional response to hypoxia.
PA1-16510 was used in western blot to identify PITX1 as a key specificity factor for HIF-1alpha dependent gene expression.
|Mudie S,Bandarra D,Batie M,Biddlestone J,Moniz S,Ortmann B,Shmakova A,Rocha S||Cell cycle (Georgetown, Tex.) (13:3878)||2015|
Systemic and Cerebral Vascular Endothelial Growth Factor Levels Increase in Murine Cerebral Malaria along with Increased Calpain and Caspase Activity and Can be Reduced by Erythropoietin Treatment.
PA1-16510 was used in western blot to study vascular endothelial growth factor signaling in mice infected with Plasmodium berghei ANKA
|Hempel C,Hoyer N,Kildemoes A,Jendresen CB,Kurtzhals JA||Frontiers in immunology (5:null)||2014|
HIF-mediated metabolic switching in bladder outlet obstruction mitigates the relaxing effect of mitochondrial inhibition.
PA1-16510 was used in western blot to study HIF-1alpha mediated metabolic switching and mitochondrial remodeling in bladder outlet obstruction
|Ekman M,Uvelius B,Albinsson S,Swärd K||Laboratory investigation; a journal of technical methods and pathology (94:557)||2014|
|Not Applicable||Not Cited||
The chromatin remodeler ISWI regulates the cellular response to hypoxia: role of FIH.
PA1-16510 was used in western blot to study the role of chromatin remodeling in the hypoxia response
|Melvin A,Mudie S,Rocha S||Molecular biology of the cell (22:4171)||2011|