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Invitrogen
HIF-2 alpha Polyclonal Antibody
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FIGURE: 1 / 5
HIF-2 alpha Antibody (PA5-79204) in WB
Western blot analysis of HIF-2A in, Lane 1: rat brain tissue lysates, Lane 2: rat RH35 whole cell lysates, Lane 3: rat small intestine tissue lysates, . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane w... View More
Product Details
PA5-79204
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human HIF-2-alpha (282-301aa, HALDSENMTKSHQNLCTKGQ).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat thymus tissue, rat testis tissue, human MCF-7 whole cell, human Hela whole cell, human Jurkat whole cell, mouse thymus tissue, mouse lun tissue, rat brain tissue, rat RH35 whole cell, rat small intestine tissue.
Target Information
HIF-2 alpha (Epas1) is a transcription factor involved in the induction of genes regulated by oxygen. HIF-2 alpha contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. It also regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of the lungs. Mutations in this gene are associated with erythrocytosis familial type 4.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Basic-helix-loop-helix-PAS protein MOP2; bHLHe73; Class E basic helix-loop-helix protein 73; Endothelial PAS domain-containing protein 1; EPAS-1; Hif like protein; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF-2 alpha; HIF-2-alpha; HIF-related factor; HIF1 alpha-like factor; HIF1alpha-like factor; HIF2 alpha; HIF2-alpha; HLF; HLF (HIF1alpha-like factor); HRF; hypoxia inducible factor 2, alpha subunit; hypoxia inducible factor 2a; hypoxia inducible transcription factor 2alpha; hypoxia-inducible factor 2 alpha; Hypoxia-inducible factor 2-alpha; Member of PAS protein 2; mHLF; PAS domain-containing protein 2; unnamed protein product
Gene Aliases: BHLHE73; ECYT4; EPAS1; HIF-2alpha; HIF2A; HLF; HRF; MOP2; PASD2
UniProt ID: (Human) Q99814, (Mouse) P97481, (Rat) Q9JHS1
Entrez Gene ID: (Human) 2034, (Mouse) 13819, (Rat) 29452
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
transcription coactivator binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific DNA binding
protein heterodimerization activity
protein dimerization activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
signal transducer activity
transcription factor binding
histone acetyltransferase binding
basic helix-loop-helix transcription factor
angiogenesis
response to hypoxia
embryonic placenta development
blood vessel remodeling
regulation of heart rate
epithelial cell maturation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
response to oxidative stress
mitochondrion organization
signal transduction
visual perception
gene expression
hemopoiesis
cell differentiation
erythrocyte differentiation
lung development
oxygen homeostasis
norepinephrine metabolic process
mRNA transcription from RNA polymerase II promoter
surfactant homeostasis
positive regulation of transcription from RNA polymerase II promoter
myoblast fate commitment
multicellular organismal iron ion homeostasis
cellular response to hypoxia
positive regulation of cold-induced thermogenesis
regulation of protein neddylation
transcription, DNA-templated
multicellular organism development
regulation of transcription from RNA polymerase II promoter in response to oxidative stress
cell maturation
iron ion homeostasis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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