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Product Details
10646-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
HIF1AN Fusion Protein Ag0997 (1-349 aa encoded by BC007719)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.13 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Product Specific Information
Immunogen sequence: MAATAAEAV ASGSGEPREE AGALGPAWDE SQLRSYSFPT RPIPRLSQSD PRAEELIENE EPVVLTDTNL VYPALKWDLE YLQENIGNGD FSVYSASTHK FLYYDEKKMA NFQNFKPRSN REEMKFHEFV EKLQDIQQRG GEERLYLQQT LNDTVGRKIV MDFLGFNWNW INKQQGKRGW GQLTSNLLLI GMEGNVTPAH YDEQQNFFAQ IKGYKRCILF PPDQFECLYP YPVHHPCDRQ SQVDFDNPDY ERFPNFQNVV GYETVVGPGD VLYIPMYWWH HIESLLNGGI TITVNFWYKG APTPKRIEYP LKAHQKVAIM RNIEKMLGEA LGNPQEVGPL LNTMIKGRYN (1-349 aa encoded by BC007719)
Target Information
Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp762F1811; Factor inhibiting HIF-1; factor inhibiting HIF1; FIH 1; FIH-1; FLJ20615; FLJ22027; hypoxia inducible factor 1, alpha subunit inhibitor; Hypoxia-inducible factor 1-alpha inhibitor; Hypoxia-inducible factor asparagine hydroxylase; peptide-aspartate beta-dioxygenase
Gene Aliases: 2310046M24Rik; A830014H24Rik; FIH; FIH1; HIF1AN
UniProt ID: (Human) Q9NWT6, (Mouse) Q8BLR9
Entrez Gene ID: (Human) 55662, (Rat) 309434, (Mouse) 319594
biological_process
peptidyl-histidine hydroxylation
peptidyl-aspartic acid hydroxylation
peptidyl-asparagine hydroxylation
positive regulation of myoblast differentiation
negative regulation of Notch signaling pathway
oxidation-reduction process
negative regulation of transcription from RNA polymerase II promoter in response to hypoxia
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter in response to hypoxia
positive regulation of vasculogenesis
regulation of transcription, DNA-templated
Notch binding
iron ion binding
protein binding
zinc ion binding
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
oxygen sensor activity
carboxylic acid binding
peptidyl-histidine dioxygenase activity
peptidyl-asparagine 3-dioxygenase activity
protein homodimerization activity
cofactor binding
NF-kappaB binding
ankyrin repeat binding
molecular_function
oxidoreductase activity
metal ion binding
dioxygenase activity
Performance Guarantee
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