Western blot analysis of HeLa Whole Cell Lysate using PA5-19456, HIF1AN primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19456 staining of Jurkat Whole Cell Lysate at a dilution of 1 ug/ml (lane 2). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
|Tested species reactivity||Chicken, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from within residues 250 to the C-terminus of Human FIH/asparagine hydroxylase.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||5 µg/ml|
|Western Blot (WB)||5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-19456 targets HIF1AN in IF and WB applications and shows reactivity with Human samples.
The PA5-19456 immunogen is synthetic peptide derived from within residues 250 to the C-terminus of Human FIH/asparagine hydroxylase.
PA5-19456 detects HIF1AN which has a predicted molecular weight of approximately 40 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.