Immunofluorescent staining of Rat PC12 cells using PA5-19494, anti-HIF2 alpha antibody. The cells were fixed with PFA (4%)for 10 minutes, permabilised PBS-T for 20 minutes and exposed to the primary antibody at a concentration of 1 ug/ml overnight at 4C. A solution of BSA (1%), normal goat serum (10%) and glycine (0.3 M) was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to Mouse HIF2-alpha aa 600-700 (internal sequence) KLH|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1 µg/ml|
|Immunofluorescence (IF)||1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:2500|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For Western Blot, this antibody detects a HIF-2-alpha specific band at ~96 kDa and has non-specific bands at 240 kDa,25 kDa and 45 kDa.
Heat mediated antigen retrieval recommended prior to tissue staining.
This antibody is predicted to react with mouse based on sequence homology.
Store antibody at 4°C for 1-2 weeks. For long-term storage, store at -20°C.
This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.