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In Western Blot analysis, a band is recognized at ~68 kDa representing HIF-3 alpha.
Because a band at ~71 kDa is also seen in hypoxic samples,it is important to run a negative control lysate as well.
Suggested positive control: Cos7 CoCl2-treated lysate.
Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1 alpha, HIF-1 beta (also designated Arnt 1), EPAS-1 (also designated HIF-2 alpha) and HIF-3 alpha, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1 alpha is upregulated and forms a heterodimer with Arnt 1 to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription.
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Protein Aliases: Basic-helix-loop-helix-PAS protein MOP7; bHLHe17; Class E basic helix-loop-helix protein 17; HIF-3-alpha; HIF3-alpha; HIF3-alpha-1; hypoxia inducible factor 3, alpha subunit; hypoxia inducible factor three alpha; Hypoxia-inducible factor 3-alpha; Inhibitory PAS domain protein; IPAS; Member of PAS protein 7; Neonatal and embryonic PAS protein; PAS domain-containing protein 7
Gene Aliases: BHLHE17; HIF3A; MOP7; Nepas; PASD7
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