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Bethyl Laboratories
HINT1 Polyclonal Antibody
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FIGURE: 1 / 1
HINT1 Antibody (A305-363A) in IP
Detection of human HINT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HINT1 antibody (Product # A305-363A) (lot 1) used for IP at 6 µg per reaction. HINT1 was also immunoprecipitated by rabbit anti-HINT1 antibody A305-373A. For blotting immunoprecipitated HINT1, A305-363A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
Product Details
A305-363A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1 to 50 of human Histidine triad nucleotide-binding protein 1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat, Bovine, Rabbit and Orangutan
Target Information
Histidine triad nucleotide-binding protein 1 (HINT1) is a member of the histidine triad (HIT) protein family, a group of small nucleotide-binding and -hydrolyzing proteins. HINT1 interacts with several diverse proteins and has been suggested to have tumor suppressive activities. HINT1 catalyzes the hydrolysis of adenosine 5'-monophoramidate substrates such as AMP-morpholidate, but its enzymatic function does not appear to play a role in its tumor suppression. Recent experiments demonstrate that HINT1 forms a complex with POSH and JNK in vivo, inhibiting AP-1 activity and the phosphorylation of c-Jun, and this action could contribute to the tumor suppressor activity of HINT1. Other studies raise the possibility of HINT1 as a candidate gene for schizophrenia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adenosine 5'-monophosphoramidase; Adenosine 5'-monophosphoramidase HINT1; Desumoylating isopeptidase HINT1; epididymis secretory sperm binding protein; FLJ30414; FLJ32340; HINT1; Histidine triad nucleotide-binding protein 1; PKCI-1; Protein kinase C inhibitor 1; Protein kinase C-interacting protein 1; unnamed protein product
Gene Aliases: HINT; HINT1; NMAN; PKCI-1; PKCI1; PRKCNH1
UniProt ID: (Human) P49773
Entrez Gene ID: (Human) 3094
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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