Western blot analysis of HCT 116 Whole Cell Lysate using PA5-19781, HINT1 primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19781 staining of Jurkat Whole Cell Lysate at a dilution of 1 ug/ml (lane 2). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HINT1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:2000|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For Western Blot, this antibody has non-specific bands at 90 kDa.
This antibody is predicted to react with mouse, rat, rabbit, cow, rhesus monkey and orangutan based on sequence homology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.