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Invitrogen
HINT1 Polyclonal Antibody
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FIGURE: 1 / 6
HINT1 Antibody (PA5-79363) in ICC/IF
Immunocytochemistry analysis of HINT1 using anti-HINT1 antibody (Product # PA5-79363) . HINT1 was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-HINT1 antibody (Product # PA5-79363) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. V... View More
Product Details
PA5-79363
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human HINT1 (59-92aa HISQISVAEDDDESLLGHLMIVGKKCAADLGLNK).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human 293T whole cell, human Jurkat whole cell, human Raji whole cell, human Hela whole cell, rat brain tissue, rat thymus tissue, mouse brain tissue, mouse thymus tissue. IHC: mouse intestine tissue, rat kidney tissue, human intestinal cancer tissue. ICC/IF: A431 cell. Flow: A549 cell.
Target Information
Histidine triad nucleotide-binding protein 1 (HINT1) is a member of the histidine triad (HIT) protein family, a group of small nucleotide-binding and -hydrolyzing proteins. HINT1 interacts with several diverse proteins and has been suggested to have tumor suppressive activities. HINT1 catalyzes the hydrolysis of adenosine 5'-monophoramidate substrates such as AMP-morpholidate, but its enzymatic function does not appear to play a role in its tumor suppression. Recent experiments demonstrate that HINT1 forms a complex with POSH and JNK in vivo, inhibiting AP-1 activity and the phosphorylation of c-Jun, and this action could contribute to the tumor suppressor activity of HINT1. Other studies raise the possibility of HINT1 as a candidate gene for schizophrenia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 17 kDa inhibitor of protein kinase C; adenosine 5'-monophosphoramidase; Adenosine 5'-monophosphoramidase HINT1; Desumoylating isopeptidase HINT1; FLJ30414; FLJ32340; Histidine triad nucleotide-binding protein 1; PKC inhibitor/ interacting protein; PKCI-1; Protein kinase C inhibitor 1; protein kinase C, iota; Protein kinase C-interacting protein 1
Gene Aliases: AA673479; HINT; HINT1; Ipk1; NMAN; Pkci; PKCI-1; PKCI1; Prkci; PRKCNH1
UniProt ID: (Human) P49773, (Mouse) P70349, (Rat) P62959
Entrez Gene ID: (Human) 3094, (Mouse) 15254, (Rat) 690660
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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