Invitrogen

HIPK2 Polyclonal Antibody

2 Published Figures

View all (12) HIPK2 antibodies

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HIPK2 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue using a HIPK2 polyclonal antibody (Product # PA5-14470), followed by HRP-conjugated secondary antibody and DAB staining.

HIPK2 Antibody in Western Blot (WB) — Western blot analysis using a HIPK2 polyclonal antibody (Product # PA5-14470) in Ramos cell lysates (35 µg per lane).

HIPK2 Antibody in Western Blot (WB) — Fig. 5 CircASH2L indirectly regulates HIPK2 via miR-129-5p in RA-FLSs. a Binding sites of miR-129-5p on HIPK2 3''UTR. b Interaction analysis between miR-129-5p and HIPK2 using the dual-luciferase reporter assay. c - f HIPK2 expression level in synovial tissues with RA and non-RA, as well as in RA-FLSs and N-FLSs was determined. g MiR-129-5p expression analysis using qRT-PCR in RA-FLSs transfected with miR-NC or miR-129-5p. h , i HIPK2 expression analysis in miR-129-5p-increased RA-FLSs with qRT-PCR and western blot. j , k RA-FLSs were co-transfected with si-circASH2L and/or anti-miR-129-5p and HIPK2 level was examined using qRT-PCR and western blot. * P <0.05

HIPK2 Antibody in Western Blot (WB) — Fig. 6 MiR-129-5p impairs RA-FLS tumor-like biologic behaviors through HIPK2. The miR-129-5p and/or HIPK2 were co-transfected into RA-FLSs. After transfection, a western blot analysis of HIPK2 expression using western blot; b , c MTT assay of cell proliferation and Edu assay; d flow cytometry of cell apoptosis detection; e western blot analysis of apoptosis-related protein expression in cells; f flow cytometry for cell cycle analysis; g , h cell migratory and invasive abilities detection using transwell assay; i the measurement of inflammatory cytokines levels using ELISA. * P <0.05

Product Details

PA5-14470

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:10-1:50	-

Product Specifications
Species Reactivity	Human
Published species	Not Applicable
Host/Isotype	Rabbit / Ig
Class	Polyclonal
Type	Antibody
Immunogen	KLH conjugated synthetic peptide between 437-468 amino acids from the N-terminal region of human HIPK2 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	2 mg/mL
Purification	Protein A, Antigen affinity chromatography
Storage buffer	PBS
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2117722

Product Specific Information

This antibody is predicted to react with mouse based on sequence homology.

Target Information

HIPK2 is a conserved serine/threonine nuclear kinase that interacts with homeodomain transcription factors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CircASH2L facilitates tumor-like biologic behaviours and inflammation of fibroblast-like synoviocytes via miR-129-5p/HIPK2 axis in rheumatoid arthritis.

Journal of orthopaedic surgery and research

Li X,Qu M,Zhang J,Chen K,Ma X

Published figure using HIPK2 polyclonal antibody (Product # PA5-14470) in Western Blot

Sat May 08 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: hHIPk2; Homeodomain-interacting protein kinase 2

Gene Aliases: HIPK2; PRO0593

UniProt ID: (Human) Q9H2X6

Entrez Gene ID: (Human) 28996

negative regulation of transcription from RNA polymerase II promoter eye development transcription, DNA-templated protein phosphorylation DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator transforming growth factor beta receptor signaling pathway smoothened signaling pathway adult walking behavior positive regulation of cell proliferation anterior/posterior pattern specification retina layer formation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation modulation by virus of host morphology or physiology neuron differentiation erythrocyte differentiation positive regulation of transforming growth factor beta receptor signaling pathway negative regulation of BMP signaling pathway PML body organization positive regulation of protein binding intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of DNA binding negative regulation of neuron apoptotic process positive regulation of angiogenesis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade embryonic camera-type eye morphogenesis voluntary musculoskeletal movement positive regulation of sequence-specific DNA binding transcription factor activity regulation of cell cycle embryonic retina morphogenesis in camera-type eye lens induction in camera-type eye SMAD protein signal transduction iris morphogenesis cellular response to hypoxia intrinsic apoptotic signaling pathway regulation of signal transduction by p53 class mediator

Process(es)

RNA polymerase II activating transcription factor binding RNA polymerase II transcription coactivator activity transcription corepressor activity protein kinase activity protein serine/threonine kinase activity protein binding ATP binding SMAD binding virion binding