Invitrogen

HLA-G Polyclonal Antibody

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HLA-G Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of HLA-G in Hela cells using a HLA-G polyclonal antibody (Product # PA5-98143) at a dilution of 1:133. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

HLA-G Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of HLA-G in paraffin embedded human placenta tissue using a HLA-G polyclonal antibody (Product # PA5-98143) at a dilution of 1:50.

HLA-G Antibody in Western Blot (WB) — Western Blot analysis of HLA-G using a HLA-G Polyclonal antibody (Product # PA5-98143) at a concentration of 8 µg/mL. Positive WB detected in: Jurkat whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 39 kDa.

Product Details

Tested Applications

Dilution

ELISA (ELISA)

Assay-Dependent

Immunocytochemistry (ICC)

1:50-1:200

Immunofluorescence (IF)

1:50-1:200

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Western Blot (WB)

1:500-1:2000

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human HLA class I histocompatibility antigen, alpha chain G protein (25-337AA)

Conjugate

Unconjugated

Form

Liquid

Concentration

4.41 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.03% Proclin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Target Information

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: b2 microglobulin; HLA class I histocompatibility antigen, alpha chain G; HLA class I molecule; HLA G antigen; HLA-G histocompatibility antigen, class I, G; MHC class I antigen G; sHLA-G; Soluble HLA class I histocompatibility antigen, alpha chain G

Gene Aliases: HLA-6.0; HLA-G; HLAG; MHC-G

UniProt ID: (Human) P17693

Entrez Gene ID: (Human) 3135

Molecular Function: cytokine receptor defense/immunity protein immunoglobulin receptor superfamily major histocompatibility complex antigen receptor