Invitrogen

HLA-G Polyclonal Antibody

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HLA-G Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HLA-G in Hela cells using a HLA-G polyclonal antibody (Product # PA5-98143) at a dilution of 1:133. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

HLA-G Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of HLA-G in paraffin embedded human placenta tissue using a HLA-G polyclonal antibody (Product # PA5-98143) at a dilution of 1:50.

HLA-G Antibody in Western Blot (WB) — Western Blot analysis of HLA-G using a HLA-G Polyclonal antibody (Product # PA5-98143) at a concentration of 8 µg/mL. Positive WB detected in: Jurkat whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 39 kDa.

Product Details

PA5-98143

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
ELISA (ELISA)	Assay-Dependent	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant Human HLA class I histocompatibility antigen, alpha chain G protein (25-337AA) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	4 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.03% Proclin 300
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2812757

Target Information

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: b2 microglobulin; DADB-15K14.8; HLA class I histocompatibility antigen, alpha chain G; HLA class I molecule; HLA G antigen; HLA-G histocompatibility antigen, class I, G; MHC class I antigen G; sHLA-G; Soluble HLA class I histocompatibility antigen, alpha chain G

Gene Aliases: HLA-6.0; HLA-G; HLAG; MHC-G

UniProt ID: (Human) P17693

Entrez Gene ID: (Human) 3135

Molecular Function: major histocompatibility complex protein