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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • HLTF Antibodies

          Invitrogen

          HLTF Polyclonal Antibody

          1 Published Figure
          1 Reference
          View all (13) HLTF antibodies

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          Cite HLTF Polyclonal Antibody

          • Antibody Testing Data (4)
          • Published Figures (1)
          HLTF Antibody in Immunocytochemistry (ICC/IF)
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          HLTF Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          HLTF Antibody (PA5-30173) in ICC/IF

          Immunocytochemistry analysis of HLTF in paraformaldehyde-fixed HeLa. Samples were incubated with HLTF polyclonal antibody (Product # PA5-30173) using a dilution of 1:500. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          HLTF Antibody in Immunocytochemistry (ICC/IF)
          HLTF Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          HLTF Antibody in Western Blot (WB)
          HLTF Antibody in Immunoprecipitation (IP)
          HLTF Antibody in Immunoprecipitation (IP)

          Product Details

          PA5-30173

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:10,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100-1:1,000
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:1,000
          -

          Immunoprecipitation (IP)

          1:100-1:500
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein encompassing a sequence within the center region of human HLTF. The exact sequence is proprietary.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.62 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          0.1M tris glycine, pH 7, with 20% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2547647

          Product Specific Information

          Recommended positive controls: HeLa.

          Predicted reactivity: Bovine (80%).

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: DNA-binding protein/plasminogen activator inhibitor 1 regulator; DNA-binding protein/plasminogen activator inhibitor-1 regulator; Helicase-like transcription factor; HIP116; mem; RING finger protein 80; RING-type E3 ubiquitin transferase HLTF; SNF2-like 3; Sucrose nonfermenting protein 2-like 3; sucrose nonfermenting-like 3; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3

          View more View less

          Gene Aliases: HIP116; HIP116A; HLTF; HLTF1; RNF80; SMARCA3; SNF2L3; ZBU1

          View more View less

          UniProt ID: (Human) Q14527

          View more View less

          Entrez Gene ID: (Human) 6596

          View more View less

          Function(s)
          RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding helicase activity protein binding ATP binding zinc ion binding ligase activity ATPase activity ubiquitin protein ligase binding poly(A) RNA binding ubiquitin protein ligase activity
          Process(es)
          protein polyubiquitination postreplication repair regulation of transcription, DNA-templated transcription from RNA polymerase II promoter covalent chromatin modification positive regulation of transcription from RNA polymerase II promoter
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
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          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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