Invitrogen

HMBS Polyclonal Antibody

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HMBS Antibody in Western Blot (WB) — Western Blot using HMBS Polyclonal Antibody (Product # PA5-117988) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: HeLa Whole Cell Lysate, Lane C: U937 Whole Cell Lysate, Lane D: K562 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 39 kDa. Observed band size: 45 kDa.

HMBS Antibody in Immunoprecipitation (IP) — HMBS Immunoprecipitation using: Lane A: 0.5 mg K562 Whole Cell Lysate 4 µL with HMBS Polyclonal Antibody (Product # PA5-117988) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: HMBS Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 39 kDa. Observed band size: 45 kDa.

Product Details

PA5-117988

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunoprecipitation (IP)	1-5 μL/mg of lysate	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E. coli-derived Human HMBS fragment
Conjugate	Unconjugated
Form	Liquid
Purification	Antigen affinity chromatography, Protein A
Storage buffer	PBS, pH 7
Contains	0.03% ProClin 300
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2902593

Target Information

PBGD (porphobilinogen deaminase), also designated hydroxymethylbilane synthase, is a cytoplasmic enzyme found in the heme synthesis pathway. PBGD belongs to the HMBS (hydroxymethylbilane synthase) family. Deficiency of PBGD causes errors in pyrrole metabolism, which in turn leads to an inherited autosomal disorder called acute intermittent porphyria (AIP). AIP is characterized by acute attacks of neurological dysfunctions with hypertension, tachycardia, peripheral neurologic disturbances, abdominal pain and excessive amounts of aminolevulinic acid and porphobilinogen in the urine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: HEM3; HMBS; Hydroxymethylbilane synthase; PBG D; PBG-D; Porphobilinogen deaminase; porphyria, acute; Chester type; Pre-uroporphyrinogen synthase; uroporphyrinogen I synthase; uroporphyrinogen I synthetase

Gene Aliases: HMBS; PBG-D; PBGD; PORC; UPS

UniProt ID: (Human) P08397

Entrez Gene ID: (Human) 3145

Molecular Function: deaminase

Function(s)

response to hypoxia protoporphyrinogen IX biosynthetic process heme biosynthetic process response to carbohydrate response to zinc ion peptidyl-pyrromethane cofactor linkage organ regeneration response to cobalt ion response to estradiol response to vitamin response to drug response to amino acid astrocyte differentiation response to methylmercury cellular response to antibiotic cellular response to arsenic-containing substance cellular response to lead ion cellular response to cytokine stimulus cellular response to amine stimulus cellular response to dexamethasone stimulus

Process(es)

hydroxymethylbilane synthase activity uroporphyrinogen-III synthase activity carboxylic acid binding amine binding coenzyme binding

HMBS Polyclonal Antibody

Product Details

PA5-117988

Western Blot (WB)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

References (0)

Bioinformatics

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