|Tested species reactivity||Dog, Human, Mouse, Pig, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 19-32 of human HMGa1.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:500|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with chicken and bovine samples based on sequence homology.
Suggested positive control: human pancreas protein, antigen standard for HMGA1 (transient overexpression lysate), pancreas lysate.
Principal Names: High Mobility Group AT-Hook 1 (HMGA-1); High mobility group protein HMG-I/HMG-Y, HMG-I(Y); HMG-R; Official Gene Symbol: HMGA1 HMGA1, an 11KDa basic, DNA-binding non-histone nuclear protein, is a key member of HMGA protein family. It consists of a highly conserved AT-hook DNA-binding domain that mediates binding to AT-rich sequences in the minor groove of chromosomal DNA. It functions as architectural chromatin-binding transcription factor altering the conformation of DNA by modulating nuclear protein-DNA complexes. It is involved in many cellular processes including growth regulation, viral induction of beta-IFN gene and regulation of inducible gene transcription. It also physically interacts with transcription factors such as AP-1, Sp-1 and CAAT/enhancer binding protein-beta to modulate their ability to activate gene expression. It functions as a direct transcriptional target of c-Jun and c-Myc and is maximally expressed during embryonic development. Pathological role of HMGA1 include integration of retroviruses into chromosomes and enhancing metastasis of tumor cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.