Invitrogen

HMGCS1 Polyclonal Antibody

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HMGCS1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of HMGCS1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGCS1 Polyclonal Antibody (Product # PA5-29488) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

HMGCS1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — HMGCS1 Polyclonal Antibody detects HMGCS1 protein at cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. HMGCS1 Polyclonal Antibody (Product # PA5-29488) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

HMGCS1 Antibody in Western Blot (WB) — Western Blot using HMGCS1 Polyclonal Antibody (Product # PA5-29488). Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HMGCS1 Polyclonal Antibody (Product # PA5-29488) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

HMGCS1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Fig. 5. PAR1 inhibition augments BDNF-mediated HMGCS1 production by murine and human neurons in vitro . Immunofluorescent (IF) images show staining for HMGCS1 (red) and neuronal marker NeuN (green) in primary murine cortical neurons treated with low-dose BDNF (1 ng/mL), the PAR1 inhibitor vorapaxar (100 nM) or a combination for 72 h. (D) The intensity of HMGCS1 in relative fluorescence units (RFU) was increased by Vora+BDNF at 72 h ( p = 0.01 compared to control, p = 0.03 relative to BDNF alone). HMGCS1 staining was also performed in human neurons, in both the SH-SY5Y cell line (B) and induced pluripotent stem cells differentiated into a spinal cord neuron phenotype (C) after treatment with vorapaxar (100 nM SH-SY5Y, 50 nM iPSC), BDNF (1 ng/mL), or combination for 72 h. Combination Vora+BDNF increased HMGCS1 fluorescence intensity relative to control and BDNF alone (D) ( p == 0.01 for each) in SH-SY5Y neurons (E) and increased HMGCS1 expression relative to control, BDNF, and vorapaxar alone ( p = 0.02, 0.01, and 0.01, respectively) in iPSC-derived human spinal cord neurons (F). (G) Increased HMGCS1 protein levels in cortical neurons with combined vorapaxar and BDNF over control or BDNF alone confirmed by Western blotting ( p = 0.03 overall, paired post-tests not statistically significant). Statistical significance determined by One-Way ANOVA on Ranks (iPSC neurons, due to unequal variance) or One-Way ANOVA (all other analyses) with Student-Newman-Keuls pairwise comparisons, *

HMGCS1 Antibody in Immunohistochemistry (IHC) — Fig. 2. PAR1 knockout promotes increased expression of cholesterol synthesis intermediates by neurons in the injured spinal cord. (A) Immunofluorescent images show co-labeling for NeuN (neuron marker) and HMGCS1 in spinal segments above the injury epicenter 30 d after 0.25 mm lateral compression or FEJOTA clip SCI in wild type (PAR1+/+) and PAR1-/- mice. The merged images show higher power views of boxed areas to demonstrate increases in neuronal HMGCS1 expression in PAR1-/- compared to controls after SCI (arrow heads indicate HMGCS1 + NeuN+ ventral horn neurons). (B) Histogram shows significant elevations in the number of HMGCS1+ neurons 30 dpi in spinal segments above and below the injury epicenter ( p < 0.001 Above, p = 0.002 Below) in LC and Above ( p = 0.04) in FEJOTA. Few HMGCS1+ neurons are present in the intact spinal cord (uninjured). (C) In contrast, the total number of neurons in the spinal cord did not significantly differ by genotype in either model. (D) Expression of HMGCS1 by ventral horn motoneurons was also documented in the intact in human spinal cord and at subacute and chronic time points after traumatic SCI. Differences between genotypes were measured by two-tailed Student's t -test (uninjured) or Two-Way Repeated Measures ANOVA with Student-Newman-Keuls pairwise comparisons (SCI), * p < 0.05. For immunostaining, n = 7 PAR1+/+ and 6 PAR1-/- female mice for LC, n = 8 PAR1+/+ and 7 PAR1-/- for FEJOTA, n = 3 each genotype for uninjured. Co-expression between

Product Details

PA5-29488

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:10,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Horse, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 194 and 455 of Human HMGCS1

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.39 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2546964

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse liver.

Predicted reactivity: Mouse (97%), Rat (97%), Pig (95%), Chicken (82%), Rhesus Monkey (99%), Bovine (96%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Transcriptional and Histochemical Signatures of Bone Marrow Mononuclear Cell-Mediated Resolution of Synovitis.

Frontiers in immunology

Menarim BC,El-Sheikh Ali H,Loux SC,Scoggin KE,Kalbfleisch TS,MacLeod JN,Dahlgren LA

PA5-29488 was used in Immunohistochemistry (Paraffin) to study differential transcriptional signatures of bone marrow mononuclear cells in response to normal and inflamed synovial fluid.

Thu Feb 10 00:00:00 EST 2022

The thrombin receptor links brain derived neurotrophic factor to neuron cholesterol production, resiliency and repair after spinal cord injury.

Neurobiology of disease

Triplet EM,Kim HN,Yoon H,Radulovic M,Kleppe L,Simon WL,Choi CI,Walsh PJ,Dutton JR,Scarisbrick IA

PA5-29488 was used in Western Blot to identify the thrombin receptor (Protease Activated Receptor 1 (PAR1)) as an integral facet of this machine with roles in regulating neurite growth through a growth factor- and cholesterol-dependent mechanism.

Sat May 01 00:00:00 EDT 2021

The thrombin receptor links brain derived neurotrophic factor to neuron cholesterol production, resiliency and repair after spinal cord injury.

Neurobiology of disease

Triplet EM,Kim HN,Yoon H,Radulovic M,Kleppe L,Simon WL,Choi CI,Walsh PJ,Dutton JR,Scarisbrick IA

Sat May 01 00:00:00 EDT 2021

The thrombin receptor links brain derived neurotrophic factor to neuron cholesterol production, resiliency and repair after spinal cord injury.

Neurobiology of disease

Triplet EM,Kim HN,Yoon H,Radulovic M,Kleppe L,Simon WL,Choi CI,Walsh PJ,Dutton JR,Scarisbrick IA

Sat May 01 00:00:00 EDT 2021

The thrombin receptor links brain derived neurotrophic factor to neuron cholesterol production, resiliency and repair after spinal cord injury.

Neurobiology of disease

Triplet EM,Kim HN,Yoon H,Radulovic M,Kleppe L,Simon WL,Choi CI,Walsh PJ,Dutton JR,Scarisbrick IA

Sat May 01 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase; 3-hydroxy-3-methylglutaryl coenzyme A synthase; 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble); 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble); HMG-CoA synthase; Hydroxymethylglutaryl-CoA synthase, cytoplasmic

Gene Aliases: B130032C06Rik; HMGCS; HMGCS1

UniProt ID: (Human) Q01581, (Mouse) Q8JZK9

Entrez Gene ID: (Human) 3157, (Mouse) 208715

Function(s)