Proteintech

HNRNPD Polyclonal Antibody

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HNRNPD Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using HNRNPD antibody 12770-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

HNRNPD Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 12770-1-AP ( HNRNPD antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

HNRNPD Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 12770-1-AP ( HNRNPD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Product Details

12770-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 16 publications 16 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

HNRNPD Fusion Protein Ag3458 (1-306 aa encoded by BC026015)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MSEEQFGGD GAAAAATAAV GGSAGEQEGA MVAATQGAAA AAGSGAGTGG GTASGGTEGG SAESEGAKID ASKNEEDEGH SNSSPRHSEA ATAQREEWKM FIGGLSWDTT KKDLKDYFSK FGEVVDCTLK LDPITGRSRG FGFVLFKESE SVDKVMDQKE HKLNGKVIDP KRAKAMKTKE PVKKIFVGGL SPDTPEEKIR EYFGGFGEVE SIELPMDNKT NKRRGFCFIT FKEEEPVKKI MEKKYHNVGL SKCEIKVAMS KEQYQQQQQW GSRGGFAGRA RGRGGDQQSG YGKVSRRGGH QNSYKPY (1-306 aa encoded by BC026015)

Target Information

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Bioinformatics

Protein Aliases: ARE-binding protein AUFI, type A; AU-rich element RNA binding protein 1, 37kDa; AU-rich element RNA-binding factor 1; AU-rich element RNA-binding protein 1; heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa); Heterogeneous nuclear ribonucleoprotein D0; hnRNP D0; RNA binding protein p45AUF1

Gene Aliases: AUF1; AUF1A; HNRNPD; hnRNPD0; HNRPD; P37

UniProt ID: (Human) Q14103, (Mouse) Q60668, (Rat) Q9JJ54

Entrez Gene ID: (Human) 3184, (Mouse) 11991, (Rat) 79256

Function(s)