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HOOK3 Polyclonal Antibody

HOOK3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

HOOK3 Antibody (15457-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using HOOK3 antibody 15457-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

HOOK3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HOOK3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

15457-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

HOOK3 Fusion Protein Ag7724 (370-718 aa encoded by BC056146)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: TYKRQVVEL QNRLSEESKK ADKLDFEYKR LKEKVDSLQK EKDRLRTERD SLKETIEELR CVQAQEGQLT TQGLMPLGSQ ESSDSLAAEI VTPEIREKLI RLQHENKMLK LNQEGSDNEK IALLQSLLDD ANLRKNELET ENRLVNQRLL EVQSQVEELQ KSLQDQGSKA EDSVLLKKKL EEHLEKLHEA NNELQKKRAI IEDLEPRFNN SSLKIEELQE ALRKKEEEMK QMEERYKKYL EKAKSVIRTL DPKQNQGAAP EIQALKNQLQ ERDRLFHSLE KEYEKTKSQR EMEEKYIVSA WYNMGMTLHK KAAEDRLAST GSGQSFLARQ RQATSSRRSY PGHVQPATAR (370-718 aa encoded by BC056146)

Target Information

Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Circ_35953 induced by the NF-κB mediated the septic AKI via targeting miR-7219-5p/HOOK3 and IGFBP7 axis.

Journal of cellular and molecular medicine

Feng Y,Liu B,Chen J,Li H,Zhang D

Mon May 01 00:00:00 UTC 2023

The mechanism of acentrosomal spindle assembly in human oocytes.

Science (New York, N.Y.)

Wu T,Dong J,Fu J,Kuang Y,Chen B,Gu H,Luo Y,Gu R,Zhang M,Li W,Dong X,Sun X,Sang Q,Wang L

Fri Nov 18 00:00:00 UTC 2022

Stem cells from human exfoliated deciduous teeth affect mitochondria and reverse cognitive decline in a senescence-accelerated mouse prone 8 model.

Cytotherapy

Guo W,Zeng Z,Xing C,Zhang J,Bi W,Yang J,Shah R,Wang D,Li Y,Zhang X,Bian Y,Du H

Sat Jan 01 00:00:00 UTC 2022

Mitotic HOOK3 phosphorylation by ERK1c drives microtubule-dependent Golgi destabilization and fragmentation.

iScience

Wortzel I,Maik-Rachline G,Yadav SS,Hanoch T,Seger R

Fri Jun 25 00:00:00 UTC 2021

The Dynein Adaptor RILP Controls Neuronal Autophagosome Biogenesis, Transport, and Clearance.

Developmental cell

Khobrekar NV,Quintremil S,Dantas TJ,Vallee RB

Mon Apr 20 00:00:00 UTC 2020

Dynein activator Hook1 is required for trafficking of BDNF-signaling endosomes in neurons.

The Journal of cell biology

Olenick MA,Dominguez R,Holzbaur ELF

Mon Jan 07 00:00:00 UTC 2019

Dynein activator Hook1 is required for trafficking of BDNF-signaling endosomes in neurons.

The Journal of cell biology

Olenick MA,Dominguez R,Holzbaur ELF

Mon Jan 07 00:00:00 UTC 2019

Hook3 is a scaffold for the opposite-polarity microtubule-based motors cytoplasmic dynein-1 and KIF1C.

The Journal of cell biology

Kendrick AA,Dickey AM,Redwine WB,Tran PT,Vaites LP,Dzieciatkowska M,Harper JW,Reck-Peterson SL

Mon Sep 02 00:00:00 UTC 2019

Hook3 is a scaffold for the opposite-polarity microtubule-based motors cytoplasmic dynein-1 and KIF1C.

The Journal of cell biology

Kendrick AA,Dickey AM,Redwine WB,Tran PT,Vaites LP,Dzieciatkowska M,Harper JW,Reck-Peterson SL

Mon Sep 02 00:00:00 UTC 2019

Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation.

Molecular & cellular proteomics : MCP

Pauwels AM,Härtlova A,Peltier J,Driege Y,Baudelet G,Brodin P,Trost M,Beyaert R,Hoffmann E

Wed May 01 00:00:00 UTC 2019

The NQO1/PKLR axis promotes lymph node metastasis and breast cancer progression by modulating glycolytic reprogramming.

Cancer letters

Yang Y,Zhu G,Dong B,Piao J,Chen L,Lin Z

Mon Jul 01 00:00:00 UTC 2019

The NQO1/PKLR axis promotes lymph node metastasis and breast cancer progression by modulating glycolytic reprogramming.

Cancer letters

Yang Y,Zhu G,Dong B,Piao J,Chen L,Lin Z

Mon Jul 01 00:00:00 UTC 2019

PTPN21 and Hook3 relieve KIF1C autoinhibition and activate intracellular transport.

Nature communications

Siddiqui N,Zwetsloot AJ,Bachmann A,Roth D,Hussain H,Brandt J,Kaverina I,Straube A

Wed Jun 19 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: h-hook3; hHK3; hook homolog 3; mHK3; Protein Hook homolog 3

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Gene Aliases: 5830454D03Rik; AI317159; E330005F07Rik; HK3; HOOK3

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UniProt ID: (Human) Q86VS8, (Mouse) Q8BUK6

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Entrez Gene ID: (Human) 84376, (Rat) 306548, (Mouse) 320191

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Function(s)

protein binding microtubule binding identical protein binding membrane traffic protein

Process(es)

endosome organization lysosome organization endosome to lysosome transport protein transport interkinetic nuclear migration cytoplasmic microtubule organization microtubule anchoring at centrosome early endosome to late endosome transport negative regulation of neurogenesis Golgi localization protein localization to centrosome neuronal stem cell population maintenance transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HOOK3 Polyclonal Antibody

HOOK3 Antibody (15457-1-AP) in ICC/IF

Product Details

15457-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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