Invitrogen

HOOK3 Polyclonal Antibody

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HOOK3 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of HOOK3 in human cerebral cortex tissue shows strong cytoplasmic positivity in neuronal cells. Samples were probed using a HOOK3 Polyclonal Antibody (Product # PA5-55172).

HOOK3 Antibody in Western Blot (WB) — Western blot analysis of HOOK3 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a HOOK3 Polyclonal Antibody (Product # PA5-55172).

HOOK3 Antibody in Immunocytochemistry (ICC/IF) — Figure 6. KIF1C recruits Hook3 to the cell periphery. (A) 293T KIF1C KO cells (KO) were infected with viral particles encoding MSCV-driven KIF1C-tagRFP-3xFLAG or KIF1C Delta794-807 -tagRFP-3xFLAG plasmids. Immunoblots were performed with the indicated antibodies. Low and high exposures with the KIF1C antibody are shown. beta-Actin provided a loading control. 293T cells transfected with CRISPR-Cas9 (CTRL) were used as control cells. (B) Confocal microscopy of KIF1C and Hook3 localization in stable 293T cell lines expressing KIF1C-tagRFP-3xFLAG or KIF1C Delta794-807 -tagRFP-3xFLAG. Cells were grown on glass coverslips, fixed, and stained for endogenous Hook3 (Endo-Hook3). The tagRFP and Hook3 signals are shown in representative maximum intensity projections. The overlap of intensity profiles (AU) generated from drawing a 15-um line segment across individual z-sections is shown to the right of the images. (C) The mean normalized Hook3 intensity within KIF1C foci for KIF1C-tagRFP-3xFLAG ( n = 25) or KIF1C Delta794-807 -tagRFP-3xFLAG ( n = 24). Foci were determined by thresholding the KIF1C image, and masks of these foci were used to measure the Hook3 intensity in the corresponding regions in maximum projection images. Box plots represent the maximum and minimum values. Statistical significance was calculated with an unpaired t test. ****, P < 0.0001. Representative data from three independent experiments is shown. (D) Confocal microscopy of KIF1C and Hook3 in U2OS cel

Product Details

PA5-55172

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (IHC)	1:50-1:200	-
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Not Applicable
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human HOOK3 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2642515

Product Specific Information

Immunogen sequence: KEEIAQRCHE LDMQVAALQE EKSSLLAENQ VLMERLNQSD SIEDPNSPAG RRHLQLQTQL EQLQEETFRL EA

Highest antigen sequence identity to the following orthologs: Mouse - 99%, Rat - 58%.

Target Information

Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.