|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide containing an N-terminal region of human HP 1 alpha (within residues 1-100).|
|Storage buffer||sodium borate|
|Storage conditions||4° C, store in dark|
This antibody is expected to react with mouse, Arabidopsis suecica, Rye and Triticum aestivum based on sequence homology.
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Antigen p25; chromobox homolog 5 (HP1 alpha homolog; chromobox homolog 5 (HP1 alpha homolog, Drosophila); Chromobox protein homolog 5; Drosophila); epididymis luminal protein 25; Heterochromatin protein 1 homolog alpha; heterochromatin protein 1-alpha; HP1 alpha; HP1 alpha homolog; HP1-ALPHA; HP1A; HP1Hs alpha
Gene Aliases: CBX5; HEL25; HP1; HP1A
UniProt ID: (Human) P45973
Entrez Gene ID: (Human) 23468
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