Proteintech

HPRT1 Polyclonal Antibody

Product Image Gallery

HPRT1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15059-1-AP (HPRT1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

HPRT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 15059-1-AP (HPRT1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

HPRT1 Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

HPRT1 Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug HPRT1 antibody (15059-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

HPRT1 Antibody in Immunoprecipitation (IP) — IP result of anti-HPRT1 (IP:15059-1-AP, 4ug; Detection:15059-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunocytochemistry (ICC)

1:10-1:100

Immunofluorescence (IF)

1:10-1:100

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:1000-1:4000

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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

HPRT1 Fusion Protein Ag7044

Conjugate

Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

The protein is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: HGPRT; HGPRTase; HPRT 1; HPRT B; hypoxanthine guanine phosphoribosyl transferase 1; hypoxanthine phosphoribosyl transferase; Hypoxanthine-guanine phosphoribosyltransferase; hypoxanthine-guanine phosphoribosyltransferase 1; testicular tissue protein Li 89

Gene Aliases: C81579; HGPRT; Hgprtase; HPGRT; HPRT; HPRT1

UniProt ID: (Human) P00492, (Mouse) P00493, (Rat) P27605

Entrez Gene ID: (Human) 3251, (Mouse) 15452, (Rat) 24465

Molecular Function: glycosyltransferase isomerase mutase transferase