Proteintech

HPRT1 Polyclonal Antibody

Product Image Gallery

HPRT1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15059-1-AP (HPRT1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

HPRT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 15059-1-AP (HPRT1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

HPRT1 Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

HPRT1 Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug HPRT1 antibody (15059-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

HPRT1 Antibody in Immunoprecipitation (IP) — IP result of anti-HPRT1 (IP:15059-1-AP, 4ug; Detection:15059-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

Product Details

15059-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	View 6 publications 6 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HPRT1 Fusion Protein Ag7044 (1-218 aa encoded by BC000578) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.29 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MATRSPGVV ISDDEPGYDL DLFCIPNHYA EDLERVFIPH GLIMDRTERL ARDVMKEMGG HHIVALCVLK GGYKFFADLL DYIKALNRNS DRSIPMTVDF IRLKSYCNDQ STGDIKVIGG DDLSTLTGKN VLIVEDIIDT GKTMQTLLSL VRQYNPKMVK VASLLVKRTP RSVGYKPDFV GFEIPDKFVV GYALDYNEYF RDLNHVCVIS ETGKAKYKA (1-218 aa encoded by BC000578 )

Target Information

The protein is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Prenatal Exposure to a Maternal High Fat Diet Increases Hepatic Cholesterol Accumulation in Intrauterine Growth Restricted Rats in Part Through MicroRNA-122 Inhibition of Cyp7a1.

Frontiers in physiology

Zinkhan EK,Yu B,Schlegel A

Wed Sep 30 00:00:00 EDT 2020

Intrauterine growth restriction combined with a maternal high-fat diet increased adiposity and serum corticosterone levels in adult rat offspring.

Journal of developmental origins of health and disease

Zinkhan EK,Yu B,Callaway CW,McKnight RA

Fri Jun 01 00:00:00 EDT 2018

Sirtuin 2 regulates cellular iron homeostasis via deacetylation of transcription factor NRF2.

The Journal of clinical investigation

Yang X,Park SH,Chang HC,Shapiro JS,Vassilopoulos A,Sawicki KT,Chen C,Shang M,Burridge PW,Epting CL,Wilsbacher LD,Jenkitkasemwong S,Knutson M,Gius D,Ardehali H

Mon Apr 03 00:00:00 EDT 2017

Intrauterine growth restriction combined with a maternal high-fat diet increases hepatic cholesterol and low-density lipoprotein receptor activity in rats.

Physiological reports

Zinkhan EK,Zalla JM,Carpenter JR,Yu B,Yu X,Chan G,Joss-Moore L,Lane RH

Fri Jul 01 00:00:00 EDT 2016

Generation of Hprt-disrupted rat through mouse←rat ES chimeras.

Scientific reports

Isotani A,Yamagata K,Okabe M,Ikawa M

Mon Apr 11 00:00:00 EDT 2016

Differential expression of interferon-γ and chemokine genes distinguishes Rasmussen encephalitis from cortical dysplasia and provides evidence for an early Th1 immune response.

Journal of neuroinflammation

Owens GC,Huynh MN,Chang JW,McArthur DL,Hickey MJ,Vinters HV,Mathern GW,Kruse CA

Thu May 02 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: HGPRT; HGPRTase; HPRT 1; HPRT B; hypoxanthine guanine phosphoribosyl transferase 1; hypoxanthine phosphoribosyl transferase; Hypoxanthine-guanine phosphoribosyltransferase; hypoxanthine-guanine phosphoribosyltransferase 1; testicular tissue protein Li 89

Gene Aliases: C81579; HGPRT; Hgprtase; HPGRT; HPRT; HPRT1

UniProt ID: (Human) P00492, (Mouse) P00493, (Rat) P27605

Entrez Gene ID: (Human) 3251, (Mouse) 15452, (Rat) 24465

Molecular Function: glycosyltransferase