Proteintech

HPRT1 Polyclonal Antibody

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HPRT1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15059-1-AP (HPRT1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

HPRT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 15059-1-AP (HPRT1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

HPRT1 Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 15059-1-AP (HPRT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

HPRT1 Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug HPRT1 antibody (15059-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

HPRT1 Antibody in Immunoprecipitation (IP) — IP result of anti-HPRT1 (IP:15059-1-AP, 4ug; Detection:15059-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

Product Details

15059-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 23 publications 23 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

HPRT1 Fusion Protein Ag7044 (1-218 aa encoded by BC000578)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MATRSPGVV ISDDEPGYDL DLFCIPNHYA EDLERVFIPH GLIMDRTERL ARDVMKEMGG HHIVALCVLK GGYKFFADLL DYIKALNRNS DRSIPMTVDF IRLKSYCNDQ STGDIKVIGG DDLSTLTGKN VLIVEDIIDT GKTMQTLLSL VRQYNPKMVK VASLLVKRTP RSVGYKPDFV GFEIPDKFVV GYALDYNEYF RDLNHVCVIS ETGKAKYKA (1-218 aa encoded by BC000578 )

Target Information

The protein is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CRISPR/Cas9-mediated generation of human embryonic stem cell sub-lines with HPRT1 gene knockout to model Lesch Nyhan disease.

Stem cell research

Boissart C,Chatrousse L,Poullion T,El-Kassar L,Giraud-Triboult K,Benchoua A

Thu Jun 15 00:00:00 EDT 2023

Mitochondrial dynamics define muscle fiber type by modulating cellular metabolic pathways.

Cell reports

Yasuda T,Ishihara T,Ichimura A,Ishihara N

Tue May 30 00:00:00 EDT 2023

Neurobehavioral deficits of mice expressing a low level of G127V mutant frataxin.

Neurobiology of disease

Fil D,Conley RL,Zuberi AR,Lutz CM,Gemelli T,Napierala M,Napierala JS

Wed Feb 01 00:00:00 EST 2023

SIRT2 inhibition protects against cardiac hypertrophy and heart failure.

bioRxiv : the preprint server for biology

Yang X,Chang HC,Tatekoshi Y,Balibegloo M,Wu R,Chen C,Sato T,Shapiro J,Ardehali H

Wed Jan 25 00:00:00 EST 2023

Arginine limitation drives a directed codon-dependent DNA sequence evolution response in colorectal cancer cells.

Science advances

Hsu DJ,Gao J,Yamaguchi N,Pinzaru A,Wu Q,Mandayam N,Liberti M,Heissel S,Alwaseem H,Tavazoie S,Tavazoie SF

Fri Jan 06 00:00:00 EST 2023

Large artery stiffening and mortality in a rat model of early vascular remodeling induced by intrauterine growth restriction and a high-fat diet.

Physiological reports

Mankouski A,Miller TA,Dodson RB,Yu B,Yang Y,Liu J,Machin DR,Donato AJ,McKnight RA,Zinkhan EK

Thu Dec 01 00:00:00 EST 2022

ZFP36L2 suppresses mTORc1 through a P53-dependent pathway to prevent peripartum cardiomyopathy in mice.

The Journal of clinical investigation

Kouzu H,Tatekoshi Y,Chang HC,Shapiro JS,McGee WA,De Jesus A,Ben-Sahra I,Arany Z,Leor J,Chen C,Blackshear PJ,Ardehali H

Mon May 16 00:00:00 EDT 2022

Guanosine primes acute myeloid leukemia for differentiation via guanine nucleotide salvage synthesis.

American journal of cancer research

Wang H,He X,Li Z,Jin H,Wang X,Li L

Wed Feb 16 00:00:00 EST 2022

Aging is associated with increased brain iron through cortex-derived hepcidin expression.

eLife

Sato T,Shapiro JS,Chang HC,Miller RA,Ardehali H

Tue Jan 11 00:00:00 EST 2022

MYC promotes cancer progression by modulating m⁶ A modifications to suppress target gene translation.

EMBO reports

Wu G,Suo C,Yang Y,Shen S,Sun L,Li ST,Zhou Y,Yang D,Wang Y,Cai Y,Wang N,Zhang H,Yang YG,Cao J,Gao P

Wed Mar 03 00:00:00 EST 2021

Genome-wide CRISPR/Cas9-knockout in human induced Pluripotent Stem Cell (iPSC)-derived macrophages.

Scientific reports

Navarro-Guerrero E,Tay C,Whalley JP,Cowley SA,Davies B,Knight JC,Ebner D

Fri Feb 19 00:00:00 EST 2021

HPRT promotes proliferation and metastasis in head and neck squamous cell carcinoma through direct interaction with STAT3.

Experimental cell research

Wang L,Wang Y,Han N,Wang X,Ruan M

Mon Feb 01 00:00:00 EST 2021

Prenatal Exposure to a Maternal High Fat Diet Increases Hepatic Cholesterol Accumulation in Intrauterine Growth Restricted Rats in Part Through MicroRNA-122 Inhibition of Cyp7a1.

Frontiers in physiology

Zinkhan EK,Yu B,Schlegel A

Wed Sep 30 00:00:00 EDT 2020

Prenatal Exposure to a Maternal High Fat Diet Increases Hepatic Cholesterol Accumulation in Intrauterine Growth Restricted Rats in Part Through MicroRNA-122 Inhibition of Cyp7a1.

Frontiers in physiology

Zinkhan EK,Yu B,Schlegel A

Wed Sep 30 00:00:00 EDT 2020

Mitochondrial damage and senescence phenotype of cells derived from a novel frataxin G127V point mutation mouse model of Friedreich's ataxia.

Disease models & mechanisms

Fil D,Chacko BK,Conley R,Ouyang X,Zhang J,Darley-Usmar VM,Zuberi AR,Lutz CM,Napierala M,Napierala JS

Mon Jul 27 00:00:00 EDT 2020

Uteroplacental Insufficiency Impairs Cholesterol Elimination in Adult Female Growth-Restricted Rat Offspring Fed a High-Fat Diet.

Reproductive sciences (Thousand Oaks, Calif.)

Zinkhan EK,Yu B,McKnight R

Sun Sep 01 00:00:00 EDT 2019

Impact of diet on the persistence of early vascular remodeling and stiffening induced by intrauterine growth restriction and a maternal high-fat diet.

American journal of physiology. Heart and circulatory physiology

Miller TA,Dodson RB,Mankouski A,Powers KN,Yang Y,Yu B,Zinkhan EK

Thu Aug 01 00:00:00 EDT 2019

Short-term Low-Dose mTORC1 Inhibition in Aged Rats Counter-Regulates Age-Related Gene Changes and Blocks Age-Related Kidney Pathology.

The journals of gerontology. Series A, Biological sciences and medical sciences

Shavlakadze T,Zhu J,Wang S,Zhou W,Morin B,Egerman MA,Fan L,Wang Y,Iartchouk O,Meyer A,Valdez RA,Mannick JB,Klickstein LB,Glass DJ

Thu Jun 14 00:00:00 EDT 2018

Intrauterine growth restriction combined with a maternal high-fat diet increased adiposity and serum corticosterone levels in adult rat offspring.

Journal of developmental origins of health and disease

Zinkhan EK,Yu B,Callaway CW,McKnight RA

Fri Jun 01 00:00:00 EDT 2018

Intrauterine growth restriction combined with a maternal high-fat diet increased adiposity and serum corticosterone levels in adult rat offspring.

Journal of developmental origins of health and disease

Zinkhan EK,Yu B,Callaway CW,McKnight RA

Fri Jun 01 00:00:00 EDT 2018

A delayed sperm penetration of cumulus layers by disruption of acrosin gene in rats.

Biology of reproduction

Isotani A,Matsumura T,Ogawa M,Tanaka T,Yamagata K,Ikawa M,Okabe M

Sat Jul 01 00:00:00 EDT 2017

Sirtuin 2 regulates cellular iron homeostasis via deacetylation of transcription factor NRF2.

The Journal of clinical investigation

Yang X,Park SH,Chang HC,Shapiro JS,Vassilopoulos A,Sawicki KT,Chen C,Shang M,Burridge PW,Epting CL,Wilsbacher LD,Jenkitkasemwong S,Knutson M,Gius D,Ardehali H

Mon Apr 03 00:00:00 EDT 2017

Sirtuin 2 regulates cellular iron homeostasis via deacetylation of transcription factor NRF2.

The Journal of clinical investigation

Yang X,Park SH,Chang HC,Shapiro JS,Vassilopoulos A,Sawicki KT,Chen C,Shang M,Burridge PW,Epting CL,Wilsbacher LD,Jenkitkasemwong S,Knutson M,Gius D,Ardehali H

Mon Apr 03 00:00:00 EDT 2017

Intrauterine growth restriction influences vascular remodeling and stiffening in the weanling rat more than sex or diet.

American journal of physiology. Heart and circulatory physiology

Dodson RB,Miller TA,Powers K,Yang Y,Yu B,Albertine KH,Zinkhan EK

Wed Feb 01 00:00:00 EST 2017

Intrauterine growth restriction combined with a maternal high-fat diet increases hepatic cholesterol and low-density lipoprotein receptor activity in rats.

Physiological reports

Zinkhan EK,Zalla JM,Carpenter JR,Yu B,Yu X,Chan G,Joss-Moore L,Lane RH

Fri Jul 01 00:00:00 EDT 2016

Intrauterine growth restriction combined with a maternal high-fat diet increases hepatic cholesterol and low-density lipoprotein receptor activity in rats.

Physiological reports

Zinkhan EK,Zalla JM,Carpenter JR,Yu B,Yu X,Chan G,Joss-Moore L,Lane RH

Fri Jul 01 00:00:00 EDT 2016

Generation of Hprt-disrupted rat through mouse←rat ES chimeras.

Scientific reports

Isotani A,Yamagata K,Okabe M,Ikawa M

Mon Apr 11 00:00:00 EDT 2016

Generation of Hprt-disrupted rat through mouse←rat ES chimeras.

Scientific reports

Isotani A,Yamagata K,Okabe M,Ikawa M

Mon Apr 11 00:00:00 EDT 2016

Combination of intrauterine growth restriction and a high-fat diet impairs cholesterol elimination in rats.

Pediatric research

Zinkhan EK,Chin JR,Zalla JM,Yu B,Numpang B,Yu X,Jiang C,Callaway CW,McKnight RA,Joss-Moore L,Lane RH

Sat Nov 01 00:00:00 EDT 2014

Differential expression of interferon-γ and chemokine genes distinguishes Rasmussen encephalitis from cortical dysplasia and provides evidence for an early Th1 immune response.

Journal of neuroinflammation

Owens GC,Huynh MN,Chang JW,McArthur DL,Hickey MJ,Vinters HV,Mathern GW,Kruse CA

Thu May 02 00:00:00 EDT 2013

Differential expression of interferon-γ and chemokine genes distinguishes Rasmussen encephalitis from cortical dysplasia and provides evidence for an early Th1 immune response.

Journal of neuroinflammation

Owens GC,Huynh MN,Chang JW,McArthur DL,Hickey MJ,Vinters HV,Mathern GW,Kruse CA

Thu May 02 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: HGPRT; HGPRTase; HPRT 1; HPRT B; hypoxanthine guanine phosphoribosyl transferase 1; hypoxanthine phosphoribosyl transferase; Hypoxanthine-guanine phosphoribosyltransferase; hypoxanthine-guanine phosphoribosyltransferase 1; testicular tissue protein Li 89

Gene Aliases: C81579; HGPRT; Hgprtase; HPGRT; HPRT; HPRT1

UniProt ID: (Human) P00492, (Mouse) P00493, (Rat) P27605

Entrez Gene ID: (Human) 3251, (Mouse) 15452, (Rat) 24465

Molecular Function: glycosyltransferase