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Invitrogen
HPRT1 Polyclonal Antibody
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FIGURE: 1 / 15
HPRT1 Antibody (PA5-22281) in ICC/IF
Immunofluorescence analysis of HPRT1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HPRT1 Rabbit Polyclonal Antibody (Product # PA5-22281) at 5 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product ... View More
Product Details
PA5-22281
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human HPRT. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.06 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, Molt-4, Raji, mouse liver.
Predicted reactivity: Rat (96%), Zebrafish (93%), Xenopus laevis (92%), Dog (96%), Pig (97%), Rabbit (98%), Chicken (94%), Chimpanzee (99%), Bovine (94%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HGPRT; HGPRTase; HPRT 1; HPRT B; hypoxanthine guanine phosphoribosyl transferase 1; hypoxanthine phosphoribosyl transferase; Hypoxanthine-guanine phosphoribosyltransferase; hypoxanthine-guanine phosphoribosyltransferase 1; testicular tissue protein Li 89
Gene Aliases: C81579; HGPRT; Hgprtase; HPGRT; HPRT; HPRT1
UniProt ID: (Human) P00492, (Mouse) P00493, (Rat) P27605
Entrez Gene ID: (Human) 3251, (Mouse) 15452, (Rat) 24465
nucleotide binding
magnesium ion binding
hypoxanthine phosphoribosyltransferase activity
protein binding
protein homodimerization activity
guanine phosphoribosyltransferase activity
transferase activity
transferase activity, transferring glycosyl groups
identical protein binding
metal ion binding
glycosyltransferase
response to amphetamine
purine nucleotide biosynthetic process
purine ribonucleoside salvage
adenine salvage
guanine salvage
grooming behavior
locomotory behavior
cytolysis
striatum development
cerebral cortex neuron differentiation
central nervous system neuron development
GMP salvage
IMP salvage
dopamine metabolic process
purine-containing compound salvage
hypoxanthine salvage
positive regulation of dopamine metabolic process
GMP catabolic process
IMP metabolic process
hypoxanthine metabolic process
lymphocyte proliferation
dendrite morphogenesis
protein homotetramerization
nucleoside metabolic process
adenine metabolic process
spermatogenesis
brain development
cellular response to insulin stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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