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Invitrogen
HRI Polyclonal Antibody
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FIGURE: 1 / 3
HRI Antibody (PA5-89632) in IHC (P)
Immunohistochemistry analysis of HRI in paraffin-embedded human liver. Samples were incubated with HRI Polyclonal antibody (Product # PA5-89632) using a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Details
PA5-89632
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 337-586 of human EIF2AK1 (NP_0552282)
Conjugate
Form
Liquid
Concentration
2.93 mg/mL
Amount
293 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: A-431, SH-SY5Y; Cellular Location: Cytoplasm
Immunogen sequence: SIVEQQLPLR RNSHLEESFT STEESSEENV NFLGQTEAQY HLMLHIQMQL CELSLWDWIV ERNKRGREYV DESACPYVMA NVATKIFQEL VEGVFYIHNM GIVHRDLKPR NIFLHGPDQQ VKIGDFGLAC TDILQKNTDW TNRNGKRTPT HTSRVGTCLY ASPEQLEGSE YDAKSDMYSL GVVLLELFQP FGTEMERAEV LTGLRTGQLP ESLRKRCPVQ AKYIQHLTRR NSSQRPSAIQ LLQSELFQNS
Target Information
EIF2AK1 (HRI) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses, leading to a decrease in protein sythesis. In the case of EIF2AK1, signaling is initiated by low levels of heme. The HRI gene is localized to 7p22 where its 3' end slightly overlaps the 3' end of the gene JTV1. The two genes are transcribed from opposite strands and kinase activity is induced by low levels of heme and inhibited by the presence of heme.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EIF-2a; EIF2a protein; Eukaryotic translation initiation factor 2-alpha kinase 1; HCR; he; heme regulated initiation factor 2 alpha kinase; heme sensitive initiation factor 2a kinase; Heme-controlled repressor; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; Heme-regulated inhibitor; heme-regulated repressor; hemin-controlled repressor; Hemin-sensitive initiation factor 2-alpha kinase; hemin-sensitive initiation factor 2a kinase; hHRI; predicted protein of HQ1362; protein kinase; unnamed protein product
Gene Aliases: EIF2AK1; HCR; hHRI; HRI; KIAA1369; LEMSPAD; PRO1362
UniProt ID: (Human) Q9BQI3, (Rat) Q63185
Entrez Gene ID: (Human) 27102, (Rat) 27137
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
eukaryotic translation initiation factor 2alpha kinase activity
protein binding
ATP binding
kinase activity
transferase activity
heme binding
protein homodimerization activity
protein serine kinase activity
non-receptor serine/threonine protein kinase
mitophagy
acute inflammatory response
translational initiation
regulation of translation
regulation of translational initiation
phagocytosis
response to stress
negative regulation of cell proliferation
negative regulation of translation
macrophage differentiation
negative regulation of translational initiation by iron
protoporphyrinogen IX metabolic process
protein autophosphorylation
regulation of hemoglobin biosynthetic process
negative regulation of hemoglobin biosynthetic process
establishment of localization in cell
multicellular organismal iron ion homeostasis
protein localization to mitochondrion
integrated stress response signaling
HRI-mediated signaling
positive regulation of mitophagy
response to iron ion starvation
protein phosphorylation
regulation of translational initiation by eIF2 alpha phosphorylation
regulation of eIF2 alpha phosphorylation by heme
negative regulation of translational initiation
iron ion homeostasis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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