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Proteintech
beta Actin Monoclonal Antibody (7D2C10), HRP
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Product Details
HRP-60008
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Plant,
Rat,
Zebrafish
Published species
Hamster,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
7D2C10
Immunogen
Recombinant protein
Conjugate
Form
Liquid
Concentration
0.33 mg/mL
Purification
Caprylic acid precipitation
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.5% BSA
Contains
0.02% thimerosal, 0.05% ProClin 300
Storage conditions
-20°C, store in dark
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
This antibody can recognize all of actins. This antibody is conjugated with HRP. Note: Do not add azide (Sodium Azide or Smite) into the dilution buffer. azide is the HRP inhibitor which decreases the enzymatic activity of HRP.
Target Information
Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Actin is a protein with a high degree of evolutionary conservation that undergoes polymerization to generate filaments, which subsequently assemble into interconnected networks within the cytoplasm of cells.; Actin, cytoplasmic 1; B-ACTZF; beta cytoskeletal actin; Beta-actin; Beta-actin-1; cytoplasmic beta-actin; I(2)-actin; PS1TP5-binding protein 1; unnamed protein product
Gene Aliases: ACTB; actba; Actx; B-ACTZF; bact; bactin1; bactzf; beta-actin; BKRNS; BNS; BRWS1; CSMH; DDS1; E430023M04Rik; PS1TP5BP1; THC8; wu:fd18f05
UniProt ID: (Human) P60709, (Rat) P60711, (Mouse) P60710, (Zebrafish) Q7ZVI7
Entrez Gene ID: (Human) 60, (Rat) 81822, (Mouse) 11461, (Zebrafish) 57934
nucleotide binding
structural constituent of cytoskeleton
protein binding
ATP binding
hydrolase activity
ATPase activity
kinesin binding
protein kinase binding
nitric-oxide synthase regulator activity
Tat protein binding
nucleosomal DNA binding
identical protein binding
tau protein binding
nitric-oxide synthase binding
structural constituent of postsynaptic actin cytoskeleton
transporter regulator activity
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
morphogenesis of a polarized epithelium
chromatin remodeling
regulation of transcription from RNA polymerase II promoter
cytoskeleton organization
establishment or maintenance of cell polarity
axonogenesis
positive regulation of cell proliferation
substantia nigra development
regulation of mitotic metaphase/anaphase transition
adherens junction assembly
maintenance of blood-brain barrier
regulation of apoptotic process
apical protein localization
positive regulation of T cell differentiation
negative regulation of cell differentiation
positive regulation of cell differentiation
positive regulation of myoblast differentiation
positive regulation of transcription, DNA-templated
cell motility
regulation of norepinephrine uptake
positive regulation of norepinephrine uptake
regulation of cell cycle
regulation of G0 to G1 transition
platelet aggregation
protein localization to adherens junction
cellular response to cytochalasin B
postsynaptic actin cytoskeleton organization
regulation of transepithelial transport
regulation of synaptic vesicle endocytosis
positive regulation of stem cell population maintenance
regulation of protein localization to plasma membrane
positive regulation of double-strand break repair via homologous recombination
regulation of G1/S transition of mitotic cell cycle
regulation of double-strand break repair
positive regulation of double-strand break repair
regulation of nucleotide-excision repair
retina homeostasis
ATP-dependent chromatin remodeling
retina development in camera-type eye
cellular response to electrical stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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