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Invitrogen
HRX Polyclonal Antibody
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FIGURE: 1 / 2
HRX Antibody (720133) in ICC/IF
Immunofluorescence was performed on fixed and permeabilized A549 cells for detection of KMT2A using Anti-KMT2A Rabbit Polyclonal Antibody (Product # 720133, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of KMT2A protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents c... View More
Product Details
720133
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Protein corresponding to Human KMT2A (aa 700-1000)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.1% BSA, 30% glycerol
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.
This antibody is predicted to react with Monkey, Equine, Pig, Bovine, Rabbit, Rat and Mouse.
Target Information
KMT2A is a histone methyltransferase that plays an essential role in early development and hematopoiesis. It is a catalytic subunit of the MLL1/MLL complex, a multiprotein complex that mediates both methylation of 'Lys-4' of histone H3 (H3K4me) complex and acetylation of 'Lys-16' of histone H4 (H4K16ac). In the MLL1/MLL complex, KMT2A specifically mediates H3K4me, a tag for epigenetic transcriptional activation. KMT2A has weak methyltransferase activity by itself, and requires other components of the MLL1/MLL complex to obtain full methyltransferase activity. It has no activity toward histone H3 phosphorylated on 'Thr-3', less activity toward H3 dimethylated on 'Arg-8' or 'Lys-9', and has higher activity toward H3 acetylated on 'Lys-9'. Required for transcriptional activation of HOXA9 and promotes PPP1R15A-induced apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acute lymphocytic 1; ALL-1; CXXC-type zinc finger protein 7; Cysteine methyltransferase KMT2A; e10e5; Histone-lysine N-methyltransferase 2A; HRX; lysine (K)-specific methyltransferase 2A; Lysine N-methyltransferase 2A; mixed lineage leukemia 1; MLL; Myeloid/lymphoid or mixed-lineage leukemia; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); Myeloid/lymphoid or mixed-lineage leukemia protein 1; Trithorax-like protein; trx Zinc-finger region homolog; unnamed protein product; Zinc finger protein HRX
Gene Aliases: ALL-1; ALL1; CXXC7; GAS7; HRX; HTRX; HTRX1; KMT2A; MLL; MLL1; MLL1A; TRX1; WDSTS
UniProt ID: (Human) Q03164
Entrez Gene ID: (Human) 4297
DNA binding
AT DNA binding
chromatin binding
protein binding
methyltransferase activity
zinc ion binding
transferase activity
histone methyltransferase activity (H3-K4 specific)
identical protein binding
protein homodimerization activity
unmethylated CpG binding
metal ion binding
protein-cysteine methyltransferase activity
histone H3K4 monomethyltransferase activity
histone H3K4 trimethyltransferase activity
immune system process
chromatin organization
regulation of transcription, DNA-templated
apoptotic process
visual learning
response to light stimulus
post-embryonic development
anterior/posterior pattern specification
regulation of gene expression
positive regulation of gene expression
methylation
circadian regulation of gene expression
embryonic hemopoiesis
exploration behavior
response to potassium ion
protein modification process
regulation of gene expression, epigenetic
T-helper 2 cell differentiation
positive regulation of gene expression, epigenetic
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
fibroblast proliferation
negative regulation of fibroblast proliferation
regulation of short-term neuronal synaptic plasticity
rhythmic process
spleen development
homeostasis of number of cells within a tissue
cognition
membrane depolarization
definitive hemopoiesis
protein-containing complex assembly
cellular response to transforming growth factor beta stimulus
negative regulation of methylation-dependent chromatin silencing
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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