Invitrogen

HS3ST2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

3 Published Figures

1 Reference

View all (3) HS3ST2 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

FIGURE: 1 / 8

HS3ST2 Antibody (PA5-26522) in WB

Western blot analysis using a HS3ST2 polyclonal antibody (Product # PA5-26522) in HeLa, 293 cell lysates (35 µg per lane).

Product Image Gallery

HS3ST2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain tissue using a HS3ST2 polyclonal antibody (Product # PA5-26522), followed by HRP-conjugated secondary antibody and DAB staining.

HS3ST2 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of HeLa cells using a HS3ST2 polyclonal antibody (Product # PA5-26522) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

HS3ST2 Antibody — Knockdown of HS3ST2 expression induced cell migration, cell invasion and cell proliferation. (A) HS3ST2 siRNA was transfected into NHBE cells, and siRNA-mediated knockdown was confirmed by Western blots. (B) Cell viability was measured by MTT assay determined by evaluating the absorbance of the converted dye at a wavelength of 570 nm. (C & D) The effect of HS3ST2 knockdown on cell migration (C) and invasion (D) was also measured in NHBE cells. NC indicates untreated normal control. {KD}

Product Details

PA5-26522

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Flow Cytometry (Flow)

1:10-1:50

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 317-346 amino acids from the C-terminal region of human HS3ST2

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2544022

Product Specific Information

This antibody is predicted to react with mouse and rat based on sequence homology.

Target Information

Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. The enzyme encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. It is a type II integral membrane protein and possesses heparan sulfate glucosaminyl 3-O-sulfotransferase activity. This gene is expressed predominantly in brain and may play a role in the nervous system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Epigenetic inactivation of heparan sulfate (glucosamine) 3-O-sulfotransferase 2 in lung cancer and its role in tumorigenesis.

PloS one

Hwang JA,Kim Y,Hong SH,Lee J,Cho YG,Han JY,Kim YH,Han J,Shim YM,Lee YS,Kim DH

PA5-26522 was used in western blot to study the prognostic value of heparin sulfate-3-O-sulfotransferase-2 epigenetic inactivation in lung cancer

Wed Sep 03 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: 3-OST-2; h3-OST-2; heparan sulfate (glucosamine) 3-O-sulfotransferase 2; heparan sulfate 3-O-sulfotransferase 2; Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 2; Heparan sulfate glucosamine 3-O-sulfotransferase 2; heparin-glucosamine 3-O-sulfotransferase

Gene Aliases: 30ST2; 3OST2; HS3ST2; UNQ2442/PRO5004

UniProt ID: (Human) Q9Y278

Entrez Gene ID: (Human) 9956

Molecular Function: transferase

Function(s)

glycosaminoglycan biosynthetic process circadian rhythm

Process(es)

sulfotransferase activity [heparan sulfate]-glucosamine 3-sulfotransferase 1 activity [heparan sulfate]-glucosamine 3-sulfotransferase 2 activity