Invitrogen

HS3ST2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

3 Published Figures

1 Reference

View all (3) HS3ST2 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

HS3ST2 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain tissue using a HS3ST2 polyclonal antibody (Product # PA5-26522), followed by HRP-conjugated secondary antibody and DAB staining.

HS3ST2 Antibody in Western Blot (WB) — Western blot analysis using a HS3ST2 polyclonal antibody (Product # PA5-26522) in HeLa, 293 cell lysates (35 µg per lane).

HS3ST2 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of HeLa cells using a HS3ST2 polyclonal antibody (Product # PA5-26522) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

HS3ST2 Antibody — Knockdown of HS3ST2 expression induced cell migration, cell invasion and cell proliferation. (A) HS3ST2 siRNA was transfected into NHBE cells, and siRNA-mediated knockdown was confirmed by Western blots. (B) Cell viability was measured by MTT assay determined by evaluating the absorbance of the converted dye at a wavelength of 570 nm. (C & D) The effect of HS3ST2 knockdown on cell migration (C) and invasion (D) was also measured in NHBE cells. NC indicates untreated normal control. {KD}

Product Details

PA5-26522

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:100	-
Flow Cytometry (Flow)	1:10-1:50	-

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / Ig
Class	Polyclonal
Type	Antibody
Immunogen	KLH conjugated synthetic peptide between 317-346 amino acids from the C-terminal region of human HS3ST2 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.4 mg/mL
Purification	Protein A, Antigen affinity chromatography
Storage buffer	PBS
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2544022

Product Specific Information

This antibody is predicted to react with mouse and rat based on sequence homology.

Target Information

Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. The enzyme encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. It is a type II integral membrane protein and possesses heparan sulfate glucosaminyl 3-O-sulfotransferase activity. This gene is expressed predominantly in brain and may play a role in the nervous system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Epigenetic inactivation of heparan sulfate (glucosamine) 3-O-sulfotransferase 2 in lung cancer and its role in tumorigenesis.

PloS one

Hwang JA,Kim Y,Hong SH,Lee J,Cho YG,Han JY,Kim YH,Han J,Shim YM,Lee YS,Kim DH

PA5-26522 was used in western blot to study the prognostic value of heparin sulfate-3-O-sulfotransferase-2 epigenetic inactivation in lung cancer

Wed Sep 03 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: 3-OST-2; h3-OST-2; heparan sulfate (glucosamine) 3-O-sulfotransferase 2; heparan sulfate 3-O-sulfotransferase 2; Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 2; Heparan sulfate glucosamine 3-O-sulfotransferase 2; heparin-glucosamine 3-O-sulfotransferase

Gene Aliases: 30ST2; 3OST2; HS3ST2; UNQ2442/PRO5004

UniProt ID: (Human) Q9Y278

Entrez Gene ID: (Human) 9956

Molecular Function: transferase