Invitrogen

HSD11B1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

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Datasheet

Protocols

Questions & Answers

Tech Support

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HSD11B1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of HSD11B1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HSD11B1 Polyclonal Antibody (Product # PA5-21586) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

HSD11B1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — HSD11B1 Polyclonal Antibody detects HSD11B1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. HSD11B1 Polyclonal Antibody (Product # PA5-21586) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

HSD11B1 Antibody in Western Blot (WB) — Western blot analysis was performed on membrane enriched cell extracts (30 µg lysate) of HeLa (Lane 1), HepG2 (Lane 2), KARPAS 299 (Lane 3), 3T3-L1 (Lane 4), 3T3-L1 differentiated to adipocytes (Lane 5), Mouse Liver (Lane 6), Rat Liver (Lane 7), Mouse Adipose (Lane 8) and Rat Adipose (Lane 9). The blot was probed with Anti-HSD11B1 Polyclonal Antibody (Product # PA5-21586, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 35 kDa band corresponding to HSD11B1 was observed in all cell lines and tissues tested and was induced upon differentiation of 3T3-L1 cells to adipocytes.

HSD11B1 Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Western blot analysis of HSD11B1 using with Anti-HSD11B1 Polyclonal Antibody (Product # PA5-21586) shows an increase in HSD11B1 upon differentiation of 3T3-L1 cells to adipocytes. {TM}

Product Details

PA5-21586

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunohistochemistry (Frozen) (IHC (F))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Sheep, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to a region within amino acids 232 and 292 of HSD11B1 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 40% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_11153989

Product Specific Information

Recommended positive controls: HepG2.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

HSD11B1 is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, HSD11B1 can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Two transcript variants encoding the same protein have been found for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 11-beta-HSD1; 11-beta-hydroxysteroid dehydrogenase 1; 11-DH; 11beta-HSD-1; 11beta-HSD1A; 11beta-hydroxysteroid dehydrogenase type 1; Corticosteroid 11-beta-dehydrogenase isozyme 1; Corticosteroid 11-beta-dehydrogenase, isozyme 1 (11-DH) (11-beta-hydroxysteroid dehydrogenase 1) (11-beta-HSD1) (11beta-HSD1A); cortocosteroid 11-beta dehydrogenase; hydroxysteroid (11-beta) dehydrogenase 1; hydroxysteroid dehydrogenase, 11 beta type 1; MGC13539; OTTHUMP00000034650; Short chain dehydrogenase/reductase family 26C member 1; short chain dehydrogenase/reductase family 26C, member 1

Gene Aliases: 11-beta-HSD1; 11-DH; CORTRD2; HDL; HSD11; HSD11B; HSD11B1; HSD11L; LRRGT00065; SDR26C1

UniProt ID: (Human) P28845, (Sheep) P51975, (Rat) P16232, (Mouse) P50172

Entrez Gene ID: (Human) 3290, (Sheep) 443409, (Rat) 25116, (Mouse) 15483

Molecular Function: dehydrogenase reverse transcriptase