Invitrogen

HSD17B1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

5 Published Figures

3 References

View all (10) HSD17B1 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

HSD17B1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (paraffin-embedded) analysis of human kidney tissue using an anti-HSD17B1 polyclonal antibody (Product # PA5-42058).

HSD17B1 Antibody in Western Blot (WB) — Western blot analysis of human Jurkat cell lysate using an anti-HSD17B1 polyclonal antibody (Product # PA5-42058).

HSD17B1 Antibody in Immunohistochemistry (IHC) — Figure 7 Effect of antagonism of TGF-beta1 (by SB431542) or of NF-kappaB (by JSH-23) on lesional development and lesional expression of steroidogenic proteins in mice with induced endometriosis. ( A ) Change in hotplate latency for mice treatment with vehicle (untreated, or U), antagonism of TGF-beta1 (T), and antagonism of NF-kappaB (N) tested at the time indicated. ( B ) Total lesion weight in the 3 groups of mice. ( C ) Boxplots of the extent of lesional platelet aggregation using CD41 the 3 groups of mice. Boxplots of lesional staining levels in the 3 groups of mice: StAR ( D ), HSD3B2 ( E ), aromatase ( F ), HSD17B1 ( G ), SF-1 ( H ), and p-CREB ( I ). The dotted horizontal line represents the median of all 3 groups. Symbols of statistical significance: *: p < 0.05, **: p < 0.01, ***: p < 0.001. NS: p > 0.05. n = 10 for each group. Except in ( A ), where the comparison was made using Kruskal's test, all tests were made using Wilcoxon's test in comparison with the untreated group ( U ). ( J ) Representative immunoreactivity staining of CD41, StAR, HSD3B2, aromatase, HSD17B1, SF-1 and p-CREB in the endometriotic lesions (x400) in Balb/c mice with induced endometriosis which then treatment with vehicle, SB431542 (an antagonist for TGF-beta1) and JSH-23 (an antagonist for NF-kappaB) for two weeks. Scale bar = 50 mum.

HSD17B1 Antibody — Figure 3 Effect of suppression of NF-kappaB activation by JSH-23 on the production of E 2 and expression of steroidogenic genes in HESCs. ( A ) Concentrations of E 2 in the supernatant of HESCs (n = 8) treated with vehicle, activated platelets and activated platelets + JSH-23. ( B ) Gene expression levels of the genes involved in estrogen biosynthesis StAR, HSD3B2, CYP19A1 and HSD17B1 in HESCs co-cultured with vehicle, activated platelets and activated platelets + JSH-23. ( C ) Representative Western blotting results for StAR, HSD3B2, aromatase and HSD17B1 protein expression. ( D ) Summary of fold changes of protein expression levels of StAR, HSD3B2, aromatase and HSD17B1 in HESCs (n = 8) treated with vehicle, activated platelets and activated platelets + JSH-23. Summary of fold changes for SF-1 ( E ) and p-CREB ( F ) protein expression in HESCs (n = 8) treated with vehicle, activated platelets and activated platelets + JSH-23. ""*"" denotes that the p-value of the difference between the groups is less than 0.05. NS: p > 0.05. Paired Wilcoxon's test was used. {TM}

Product Details

PA5-42058

Applications	Tested Dilution	Publications
Western Blot (WB)	0.2-1 µg/mL	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	4-8 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide directed towards the N-terminal of human HSD17B1 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS with 2% sucrose
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2609429

Product Specific Information

Peptide sequence: MARTVVLITG CSSGIGLHLA VRLASDPSQS FKVYATLRDL KTQGRLWEAA

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 91%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%

Target Information

HSD17B1 is favors the reduction of estrogens and androgens. It also has 20-alpha-HSD activity. It uses preferentially NADH.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Estrogen biosynthesis in cultured skeletal muscle cells (L6) induced by amino acids.

Genes & nutrition

Iresjö BM,Landin A,Ohlsson C,Lundholm K

PA5-42058 was used in Western Blotting to investigate if increased availability of amino acids induced biosynthesis of sex steroids in skeletal muscles.

Mon Apr 11 00:00:00 EDT 2022

Platelets induce increased estrogen production through NF-κB and TGF-β1 signaling pathways in endometriotic stromal cells.

Scientific reports

Qi Q,Liu X,Zhang Q,Guo SW

Published figure using HSD17B1 polyclonal antibody (Product # PA5-42058) in Immunohistochemistry

Tue Jan 28 00:00:00 EST 2020

Platelets induce increased estrogen production through NF-κB and TGF-β1 signaling pathways in endometriotic stromal cells.

Scientific reports

Qi Q,Liu X,Zhang Q,Guo SW

Published figure using HSD17B1 polyclonal antibody (Product # PA5-42058) in Immunohistochemistry

Tue Jan 28 00:00:00 EST 2020

Bioinformatics

Protein Aliases: 17-beta-HSD 1; 17-beta-hydroxysteroid dehydrogenase type 1; 20 alpha-hydroxysteroid dehydrogenase; 20-alpha-HSD; E2DH; Estradiol 17-beta-dehydrogenase 1; estradiol 17-beta-dehydrogenase-1; hydroxysteroid (17-beta) dehydrogenase 1 isoform; Placental 17-beta-hydroxysteroid dehydrogenase; Short chain dehydrogenase/reductase family 28C member 1; short chain dehydrogenase/reductase family 28CE, member 1

Gene Aliases: 17beta-HSD; 17HSDB1; E17KSR; E2DH; EDH17B1; EDH17B2; EDHB17; HSD17; HSD17B1; Hsd17ba; SDR28C1

UniProt ID: (Human) P14061, (Mouse) P51656

Entrez Gene ID: (Human) 3292, (Mouse) 15485

Molecular Function: dehydrogenase