Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:1,000 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Synthetic peptide corresponding to residues in Human HSD3B2. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.2, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2719326 |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
3 beta-hydroxysteroid dehydrogenase (3beta-HSD), also known as HSD3B1 or HSDB3, is a bifunctional enzyme that plays a crucial role in the synthesis of all classes of hormonal steroids. Two human 3beta-HSD proteins, designated type I (3beta-HSD) and type II (3beta-HSD2), are expressed by different genes and function in different areas of the body. Localized to the membrane of the endoplasmic reticulum (ER) and expressed in skin and placenta, 3beta-HSD is the type I protein that catalyzes the oxidative conversion of delta5-ene-3-beta-hydroxy steroid, as well as the conversion of various ketosteroids. Defects in the gene encoding 3beta-HSD are associated with classic salt wasting, genital ambiguity, hypogonadism, insulin-resistant polycystic ovary syndrome (PCOS) and an increased susceptibility to prostate cancer. Additionally, congenital deficiency of 3beta-HSD activity results in a severe depletion of steroid formation which can be lethal in young children.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 3 beta HSD II; 3 beta-HSD type II; 3 beta-hydroxysteroid dehydrogenase 2; 3 beta-hydroxysteroid dehydrogenase 6; 3 beta-hydroxysteroid dehydrogenase type II, delta 5-delta 4-isomerase type II, 3 beta-HSD type II; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 4; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type IV; 3-beta-HSD adrenal and gonadal type; 3-beta-HSD II; 3-beta-HSD IV; 3-beta-hydroxy-5-ene steroid dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3beta-HSD; delta 5-delta 4-isomerase type II; Delta-5-3-ketosteroid isomerase; HSD-3beta; Hydroxy-delta-5-steroid dehydrogenase 3 beta- and steroid delta-isomerase; hydroxysteroid dehydrogenase-2, delta<5>-3-beta; hydroxysteroid dehydrogenase-6 delta<5>-3-beta; Progesterone reductase; short chain dehydrogenase/reductase family 11E, member 2; Steroid Delta-isomerase
Gene Aliases: HSD3B; Hsd3b1; HSD3B2; Hsd3b6; HSDB; HSDB3B; SDR11E2
UniProt ID: (Human) P26439, (Mouse) P26149, (Rat) Q62878
Entrez Gene ID: (Human) 3284, (Mouse) 15493, (Rat) 29632
Molecular Function:
dehydratase
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support
