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Invitrogen

HSF1 Polyclonal Antibody

4 Published Figures
22 References
View all (55) HSF1 antibodies

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Product Details

PA3-017

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:10,000
View 14 publications 14 publications

Immunocytochemistry (ICC/IF)

1:50
View 2 publications 2 publications

Flow Cytometry (Flow)

-
View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent
-

Immunoprecipitation (IP)

2 µL
-

Neutralization (Neu)

-
View 1 publication 1 publication

Gel Shift (EMSA)

Assay-dependent
View 4 publications 4 publications
Product Specifications

Species Reactivity

C. elegans, Human, Mouse

Published species

Human, Mollusc, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant human HSF1 expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

PBS, whole serum

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325968

Product Specific Information

PA3-017 detects heat shock factor 1 (HSF-1) from human, mouse and rat tissues and cells.

PA3-017 has been successfully used in Western blot, immunoprecipitation, immunofluorescence, ELISA, and gel super shift protocols. By Western blot, this antibody detects an ~83 kDa protein representing HSF-1 from NIH-3T3 cell lysate.

The PA3-017 immunogen is recombinant human HSF-1 protein expressed in E. coli.

Target Information

All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Heat shock factor protein 1; Heat shock transcription factor 1; heat shock transcription factor 1 alpha isoform; heat shock transcription factor 1 beta isoform; heat shock transcription factor 1 gammaalpha isoform; heat shock transcription factor 1 gammabeta isoform; HSF 1; HSF1; Hsf1gammaalpha; Hsf1gammabeta; HSTF 1; unnamed protein product

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Gene Aliases: AA960185; HSF1; Hsf1alpha; Hsf1beta; HSTF1

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UniProt ID: (Human) Q00613, (Mouse) P38532

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Entrez Gene ID: (Human) 3297, (Mouse) 15499

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding protein kinase binding heat shock protein binding chromatin DNA binding identical protein binding sequence-specific DNA binding protein heterodimerization activity Hsp90 protein binding translation elongation factor binding STAT family protein binding sequence-specific single stranded DNA binding general transcription initiation factor binding sequence-specific double-stranded DNA binding promoter-specific chromatin binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding winged helix/forkhead transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter MAPK cascade in utero embryonic development embryonic placenta development DNA repair regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter mRNA processing defense response cellular response to DNA damage stimulus female meiotic division spermatogenesis response to nutrient cell proliferation response to heat positive regulation of gene expression negative regulation of gene expression negative regulation of cardiac muscle cell apoptotic process response to activity negative regulation of protein complex assembly response to estradiol response to lipopolysaccharide negative regulation of tumor necrosis factor production response to testosterone cellular response to heat cellular response to unfolded protein cellular response to potassium ion positive regulation of multicellular organism growth response to amino acid positive regulation of macrophage differentiation positive regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter epithelial cell proliferation negative regulation of epithelial cell proliferation mRNA transport embryonic process involved in female pregnancy positive regulation of stress granule assembly protein-containing complex assembly cellular response to hydrogen peroxide cellular response to lipopolysaccharide cellular response to amino acid stimulus cellular response to cadmium ion cellular response to copper ion cellular response to estradiol stimulus cellular response to xenobiotic stimulus cellular response to radiation cellular response to gamma radiation cellular response to diamide negative regulation of inclusion body assembly positive regulation of inclusion body assembly positive regulation of cold-induced thermogenesis regulation of cellular response to heat response to peptide positive regulation of apoptotic DNA fragmentation cellular response to sodium arsenite cellular response to angiotensin cellular response to nitroglycerin cellular response to L-glutamine response to hypobaric hypoxia response to psychosocial stress negative regulation of double-strand break repair via nonhomologous end joining positive regulation of apoptotic signaling pathway transcription, DNA-templated transcription from RNA polymerase II promoter protein phosphorylation negative regulation of cell proliferation mRNA transcription regulation of spindle checkpoint
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