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Invitrogen
HSF2 Polyclonal Antibody
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FIGURE: 1 / 1
HSF2 Antibody (PA5-88454) in WB
Western blot analysis of HSF2 in extracts of various cell lines. Samples were incubated with HSF2 Polyclonal antibody (Product # PA5-88454) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Product Details
PA5-88454
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 377-536 of human HSF2 (NP_0044971)
Conjugate
Form
Liquid
Concentration
0.74 mg/mL
Amount
74 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MLSGRQFSID PDLLVDLFTS SVQMNPTDYI NNTKSENKGL ETTKNNVVQP VSEEGRKSKS KPDKQLIQYT AFPLLAFLDG NPASSVEQAS TTASSEVLSS VDKPIEVDEL LDSSLDPEPT QSKLVRLEPL TEAEASEATL FYLCELAPAP LDSDMPLLDS; Positive Samples: U-87MG, LO2, HeLa, Mouse heart, Rat liver, Rat testis; Cellular Location: Cytoplasm, Nucleus
Target Information
Prokaryotic and eukaryotic cells respond to thermal and chemical stress by inducing a group of genes collectively designated heat shock genes. In eukaryotes, this gene expression is regulated primarily at the transcription level. Heat shock transcription factors (HSF, also designated HSTF) 1 and 2 are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein level. HSF1 is normally found as a monomer whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development, and, like HSF1, binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: heat shock factor 2; Heat shock factor protein 2; Heat shock transcription factor 2; HSF 2; HSTF 2; MGC117376; MGC156196; MGC75048; unnamed protein product
Gene Aliases: AI661205; HSF 2; HSF2; HSTF 2; HSTF2
UniProt ID: (Human) Q03933, (Mouse) P38533
Entrez Gene ID: (Human) 3298, (Mouse) 15500, (Rat) 64441
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
identical protein binding
sequence-specific DNA binding
sequence-specific double-stranded DNA binding
protein homodimerization activity
winged helix/forkhead transcription factor
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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