Invitrogen

HSP105 Polyclonal Antibody

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HSP105 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of HSP105 in paraffin-embedded human breast carcinoma tissue. Samples were incubated with HSP105 polyclonal antibody (Product # PA5-36059) at a dilution of 1:100.

HSP105 Antibody in Western Blot (WB) — Western blot analysis of HSP105 in Lane 1: MCF-7 whole cell lysate (40 µg), Lane 2: PC12 whole cell lysate (40 µg), Lane 3: the lung tissue lysate of mouse (30 µg), Lane 4: the lung tissue lysate of rat (30 µg). Samples were incubated with HSP105 polyclonal antibody (Product # PA5-36059) at a dilution of 1:1,000.

HSP105 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of HSP105 in L02 cell lysate (sepharose bead conjugate, lane 2 and lane 3) and Nonspecific IgG Control (sepharose bead conjugate, lane 4). Lane 1 is 20% input. Samples were incubated with HSP105 polyclonal antibody (Product # PA5-36059) Results legends show supernatant (upward arrow) and deposition (downward arrow).

Product Details

PA5-36059

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
Immunoprecipitation (IP)	1:10-1:100	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to amino acids 780-830 of human HSP105 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2553349

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 105 kDa.

Purity is >95% by SDS-PAGE.

Target Information

HSPH1 prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. This protein inhibits HSPA8/HSC70 ATPase and chaperone activities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 42 degrees C-HSP; Antigen NY-CO-25; heat shock 105kD alpha; heat shock 105kD beta; heat shock 105kDa; heat shock 105kDa/110kDa protein 1; Heat shock 110 kDa protein; Heat shock protein; Heat shock protein 105 kDa; heat shock protein 110; heat shock protein, 105 kDa; Heat shock-related 100 kDa protein E7I; HSP; HSP-E7I; HSP105 42 C-HSP; NY CO 25

Gene Aliases: 105kDa; AI790491; hsp-E7I; HSP105; HSP105A; HSP105B; HSP110; hsp110/105; HSPH1; KIAA0201; NY-CO-25

UniProt ID: (Human) Q92598, (Mouse) Q61699, (Rat) Q66HA8

Entrez Gene ID: (Human) 10808, (Mouse) 15505, (Rat) 288444

Function(s)