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Invitrogen
HSP27 Polyclonal Antibody
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FIGURE: 1 / 5
HSP27 Antibody (AHO1132) in WB
Western blot was performed using Anti-HSP27 Polyclonal Antibody(Product # AHO1132) and a 25kDa band corresponding to HSP27 was observed across cell lines tested except HT-1080 which is reported to be negative. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), MDA-MB-231 (Lane 2), PC-3 (Lane 3), DU 145 (Lane 4), HeLa (Lane 5), LNCaP (Lane 6) and HT-1080 (Lane 7) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by i... View More
Product Details
AHO1132
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant full length human HSP27 expressed in E. coli.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
purified
Storage buffer
PBS, pH 7.2, with 1% BSA
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 27-kda heat shock protein; 28 kDa heat shock protein; DKFZp586P1322; epididymis secretory protein Li 102; Estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; Heat shock 25 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; Heat shock protein; Heat shock protein beta-1; Heat shock protein family B member 1; heat shock protein, 25 kDa; HS; HSP; HSP 25; HSP 27; HSP27; HSPB1; p25; Phospho-HSP 27; SRP27; Stress-responsive protein 27; truncated hsp25; unnamed protein product
Gene Aliases: 27kDa; CMT2F; HEL-S-102; HMN2B; HMND3; HS.76067; Hsp25; HSP27; HSP28; HSPB1; SRP27
UniProt ID: (Human) P04792, (Mouse) P14602
Entrez Gene ID: (Human) 3315, (Mouse) 15507, (Rat) 24471
RNA binding
protein kinase C binding
protein binding
protein kinase C inhibitor activity
protein kinase binding
identical protein binding
protein homodimerization activity
ubiquitin binding
protein binding involved in protein folding
unfolded protein binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
poly(A) RNA binding
regulation of protein phosphorylation
regulation of translational initiation
protein folding
response to unfolded protein
response to heat
response to virus
regulation of autophagy
positive regulation of interleukin-1 beta production
positive regulation of tumor necrosis factor production
intracellular signal transduction
cellular response to vascular endothelial growth factor stimulus
protein refolding
negative regulation of apoptotic process
regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
vascular endothelial growth factor receptor signaling pathway
platelet aggregation
negative regulation of protein kinase C signaling
anterograde axonal protein transport
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
positive regulation of endothelial cell chemotaxis
negative regulation of apoptotic signaling pathway
negative regulation of protein kinase activity
positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway
positive regulation of tumor necrosis factor biosynthetic process
negative regulation of protein serine/threonine kinase activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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