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Invitrogen
HSP27 Polyclonal Antibody
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FIGURE: 1 / 7
HSP27 Antibody (PA5-85351) in ICC/IF
HSP27 Polyclonal Antibody detects HSP27 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSP27 protein stained by HSP27 Polyclonal Antibody (Product # PA5-85351) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-85351
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human HSP27. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.82 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (84%), Rat (84%), Dog (88%), Pig (89%), Rhesus Monkey (100%), Bovine (86%).
Positive Control: 293T, A431, HeLa, HepG2, HT-29.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 28 kDa heat shock protein; DKFZp586P1322; epididymis secretory protein Li 102; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; Heat shock protein; Heat shock protein beta-1; Heat shock protein family B member 1; HS; HSP; HSP 27; HspB1; Phospho-HSP 27; SRP27; Stress-responsive protein 27
Gene Aliases: CMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; HSP27; HSP28; HSPB1; SRP27
UniProt ID: (Human) P04792
Entrez Gene ID: (Human) 3315
retina homeostasis
regulation of translational initiation
negative regulation of protein kinase activity
movement of cell or subcellular component
response to unfolded protein
response to virus
regulation of autophagy
positive regulation of interleukin-1 beta production
intracellular signal transduction
cellular response to vascular endothelial growth factor stimulus
positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway
positive regulation of tumor necrosis factor biosynthetic process
negative regulation of apoptotic process
regulation of I-kappaB kinase/NF-kappaB signaling
regulation of mRNA stability
positive regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
vascular endothelial growth factor receptor signaling pathway
platelet aggregation
negative regulation of protein serine/threonine kinase activity
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
positive regulation of endothelial cell chemotaxis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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