Invitrogen

HSP40 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

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Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

HSP40 Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of HSP40 was performed using 70% confluent log phase HeLa cells heat shocked at 43°C for 1 hr. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HSP40 Polyclonal Antibody (Product # PA5-17382) at 1:400 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear translocation upon heat shock. Panel e shows untreated HeLa cells with cytoplasmic localization. Panel f represents control cells with no primary to assess background. The images were captured at 60X magnification.

HSP40 Antibody in Western Blot (WB) — Knockdown of HSP40 was achieved by transfecting HeLa cells with HSP40 specific siRNAs (Silencer® select Product # s7009, s223882). Western blot analysis (Fig. a) was performed using whole cell extracts from the HSP40 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with HSP40 Polyclonal Antibody (Product # PA5-17382, 1:2000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to HSP40.

HSP40 Antibody in Cell treatment — Altered expression and translocation of target protein upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis of HSP40 using with HSP40 Polyclonal Antibody (Product # PA5-17382) shows a translocation to nucleus upon heat shock treatment. {TM}

HSP40 Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with HSP40 specific siRNA and decrease of signal was observed in Western Blot using Anti-HSP40 Polyclonal Antibody (Product # PA5-17382). {KD}

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1:200

Immunocytochemistry (ICC)

1:400

Immunofluorescence (IF)

1:400

Immunoprecipitation (IP)

1:50

Western Blot (WB)

1:1000

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to Glu300 of human HSP40/Hdj1

Conjugate

Unconjugated

Form

Liquid

Concentration

35 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

RRID

AB_10984351

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other HSPs.

Target Information

TCF-9 (transcription factor 9), also known as C2orf3 (chromosome 2 open reading frame 3), GCF (GC-rich sequence DNA-binding factor) or DNABF, is a 781 amino acid nuclear protein that belongs to the GCF family. Widely expressed, GCF binds the GC-rich sequences of beta-Actin, EGFR and calcium-dependent protease (CANP) promoters. GCF contains multiple phosphoserine and phosphothreonine residues, and two GCF isoforms are produced due to alternative splicing events. GCF is considered a candidate for susceptibility to dyslexia (DYX3) as both genes reside in close proximity on human chromosome 2. Chromosome 2 is the second largest human chromosome and consists of 237 million bases, encodes over 1,400 genes and makes up approximately 8% of the human genome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DnaJ (Hsp40) homolog, subfamily B, member 1; DnaJ homolog subfamily B member 1; DnaJ protein homolog 1; hDj-1; Heat shock 40 kDa protein 1; Heat shock protein; HSP; HSP 40; HSP40; Human DnaJ protein 1; NDAJB1; radial spoke 16 homolog B

Gene Aliases: 0610007I11Rik; DjB1; DNAJ1; DNAJB1; HDJ1; Hsp40; HSPF1; RSPH16B; Sis1

UniProt ID: (Human) P25685, (Mouse) Q9QYJ3

Entrez Gene ID: (Human) 3337, (Rat) 361384, (Mouse) 81489

Molecular Function: chaperone chaperonin