Invitrogen

HSP40 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

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Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

HSP40 Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of HSP40 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HSP40 Rabbit Polyclonal Antibody (Product # PA5-76452) at 5 microgram/ml in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

HSP40 Antibody in Western Blot (WB) — Western blot was performed using Anti-HSP40 Polyclonal Antibody (Product # PA5-76452) and a 40 kDa band corresponding to HSP40 was observed across cell lines and tissue extract tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), U-87 MG (Lane 2), MCF-7 (Lane 3), Caco-2 (Lane 4), Hep G2 (Lane 5) and tissue extract of Mouse Testis (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

HSP40 Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with HSP40 siRNA and decrease in signal intensity was observed in western blot application using HSP40 Polyclonal Antibody (Product # PA5-76452). {KD}

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:50-1:200

Immunofluorescence (IF)

1:50-1:200

Western Blot (WB)

1:500-1:2000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant full length Human HSP40.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2720179

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Target Information

TCF-9 (transcription factor 9), also known as C2orf3 (chromosome 2 open reading frame 3), GCF (GC-rich sequence DNA-binding factor) or DNABF, is a 781 amino acid nuclear protein that belongs to the GCF family. Widely expressed, GCF binds the GC-rich sequences of beta-Actin, EGFR and calcium-dependent protease (CANP) promoters. GCF contains multiple phosphoserine and phosphothreonine residues, and two GCF isoforms are produced due to alternative splicing events. GCF is considered a candidate for susceptibility to dyslexia (DYX3) as both genes reside in close proximity on human chromosome 2. Chromosome 2 is the second largest human chromosome and consists of 237 million bases, encodes over 1,400 genes and makes up approximately 8% of the human genome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DnaJ (Hsp40) homolog, subfamily B, member 1; DnaJ homolog subfamily B member 1; DnaJ protein homolog 1; hDj-1; Heat shock 40 kDa protein 1; Heat shock protein; HSP; HSP 40; HSP40; Human DnaJ protein 1; NDAJB1; radial spoke 16 homolog B

Gene Aliases: 0610007I11Rik; DjB1; DNAJ1; DNAJB1; HDJ1; Hsp40; HSPF1; RSPH16B; Sis1

UniProt ID: (Human) P25685, (Mouse) Q9QYJ3

Entrez Gene ID: (Human) 3337, (Rat) 361384, (Mouse) 81489

Molecular Function: chaperone chaperonin