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HSP701L Antibody (PA5-28202) in WB
Western Blot using HSP701L Polyclonal Antibody (Product # PA5-28202). Sample (30 µg whole cell lysate). A: H1299. B: HeLa S3. C: HepG2. D: MOLT4. E: Raji. 7.5% SDS PAGE. HSP701L Polyclonal Antibody (Product # PA5-28202) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Product Details
PA5-28202
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 335 and 639 of Human HSP70 1L
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2545678
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, mouse testis, rat testis, U87-MG, SK-N-SH, IMR32, SK-N-AS.^M
^M
Predicted reactivity: Mouse (93%), Rat (92%), Xenopus laevis (81%), Pig (94%), Bovine (94%).^M
^M
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock proteins. One class of stress proteins, termed the "small heat shock proteins" is comprised of a diverse group of proteins from approximately15 to >30 kDa, with a single form found in yeast and human cells and multiple related forms found in higher plants. A direct relationship between elevated strwess protein expression and neoplasia has been suggested from studies on estrogen-induced proteins in breast cancer cells. In addition, Hsp27 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoter (e. g. phorbol esters) and calcium ionophores. The expression of a 24 kDa protein was shown to correlate with steroid hormone receptor in cell lines, organs, and tumors. The 24 kDa estrogen-induced protein was found to be expressed at high levels in carcinoma of the breast. Another study has suggested that levels of the Hsp27 correlate with endometrial adenocarcinomas. Interestingly, the regulation of human Hsp27 by estrogens and heat shock is reminiscent of the ecdysteroid and heat shock activation of the drosophila Hsp20 genes.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: heat shock 10kDa protein 1-like; Heat shock 70 kDa protein 1-Hom; Heat shock 70 kDa protein 1-like; Heat shock 70 kDa protein 1L; Heat shock 70 kDa protein 3; Heat shock 70 kDa-like protein 1; heat shock 70kD protein-like 1; heat shock 70kDa protein 1-like; Heat shock protein; Heat shock protein 70-1l; heat shock protein cognate 70, testis; Heat shock protein family A member 1L; heat shock-related protein hsc70t; HSP70-Hom; HSP70.3; inducible heat shock protein 70; Spermatid-specific heat shock protein 70
Gene Aliases: Hsc70t; HSP70-1L; Hsp70-3; HSP70-HOM; HSP70T; HSPA1L; hum70t; Msh5
UniProt ID: (Human) O75634, (Rat) P55063, (Mouse) P16627
Entrez Gene ID: (Human) 3305, (Rat) 24963, (Mouse) 15482
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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