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Invitrogen
HSP90 beta Polyclonal Antibody
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FIGURE: 1 / 29
HSP90 beta Antibody (PA3-012) in ICC/IF
Immunofluorescent analysis of Heat Shock Protein 84 using Anti-Heat Shock Protein 84 Polyclonal Antibody (Product # PA3-012) shows staining in U251 Cells. Heat Shock Protein 84 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibo... View More
Product Details
PA3-012
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
C. elegans,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues P(2) E E V H H G E E E V E(13) of mouse HSP84.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA3-012 detects heat shock protein 90 beta (HSP90) from human, monkey, mouse, and rat tissues and cells. This antibody does not detect HSP86.
PA3-012 has been successfully used in Western blot, immunohistochemistry, immunofluorescence, flow cytometry, and immunoprecipitation procedures. By Western blot, this antibody detects an ~84 kDa protein representing HSP84 from Hepa1 cell lysate. Immunofluorescence staining of HSP84 in Hepa1 cells with PA3-012 yields a pattern consistent with cytoplasmic staining. Immunoprecipitation experiments with this antibody suggest that HSP84 exists primarily as homodimers in Hepa 1 cells. Furthermore, the antibody is capable of precipitating HSP84 that is complexed with other proteins such as the aryl hydrocarbon (Ah) receptor.
The PA3-012 immunogen is a synthetic peptide corresponding to residues P(2) E E V H H G E E E V E(13) of mouse HSP84. The N-terminal regions of HSP84 and HSP86 show the largest difference in amino acid sequence.
Target Information
Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, aryl hydrocarbon (Ah) receptor, and some kinases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Heat shock 84 kDa; heat shock 90kD protein 1, beta; heat shock 90kDa protein 1, beta; Heat shock protein; heat shock protein 1, beta; heat shock protein 90 kDa; heat shock protein 90kDa alpha (cytosolic), class B member 1; heat shock protein 90kDa alpha family class B member 1; Heat shock protein family C member 3; Heat shock protein HSP 90-beta; heat shock protein, 84 kDa 1; HSP; HSP 84; HSP 90; HSP84; HSP90-beta; retinal degeneration slow protein; RP1-302G2.1; TSTA; Tumor-specific transplantation 84 kDa antigen
Gene Aliases: 90kDa; AL022974; C81438; D6S182; HSP84; Hsp84-1; Hsp90; HSP90-BETA; HSP90AB1; HSP90B; HSPC2; HSPC3; HSPCB
UniProt ID: (Human) P08238, (Mouse) P11499, (Rat) P34058
Entrez Gene ID: (Human) 3326, (Mouse) 15516, (Rat) 301252
UTP binding
CTP binding
double-stranded RNA binding
protein binding
ATP binding
GTP binding
sulfonylurea receptor binding
protein kinase regulator activity
kinase binding
protein kinase binding
MHC class II protein complex binding
nitric-oxide synthase regulator activity
TPR domain binding
dATP binding
histone deacetylase binding
ion channel binding
poly(A) RNA binding
unfolded protein binding
cadherin binding involved in cell-cell adhesion
nucleotide binding
Hsp90 family chaperone
placenta development
protein folding
response to unfolded protein
response to salt stress
virion attachment to host cell
regulation of protein ubiquitination
positive regulation of protein binding
negative regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of phosphoprotein phosphatase activity
positive regulation of protein import into nucleus, translocation
Fc-gamma receptor signaling pathway involved in phagocytosis
negative regulation of neuron apoptotic process
positive regulation of nitric oxide biosynthetic process
positive regulation of cell size
protein stabilization
regulation of interferon-gamma-mediated signaling pathway
regulation of type I interferon-mediated signaling pathway
cellular response to interleukin-4
cellular response to organic cyclic compound
positive regulation of protein serine/threonine kinase activity
fibril organization
cell-cell adhesion
regulation of cellular response to heat
response to stress
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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