Invitrogen

HSP90 beta Polyclonal Antibody

3 References

View all (28) HSP90 beta antibodies

Datasheet

Protocols

Tech Support

Datasheet

Protocols

Tech Support

Product Image Gallery

HSP90 beta Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Heat Shock Protein 90 beta (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) a Heat Shock Protein 90 beta polyclonal antibody (Product # PA5-16327) at a dilution of 1:200 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or a ToxInsight Instrument at 20X magnification.

HSP90 beta Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), NIH/3T3 (Lane 2), PC-3 (Lane 3), LNCaP (Lane 4), K-562 (Lane 5), A549 (Lane 6), HT-1080 (Lane 7) and HEK 293 (Lane 8). The blot was probed with Rabbit Anti-HSP90 Polyclonal Antibody (Product # PA5-16327, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 90 kDa band corresponding to HSP90 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

HSP90 beta Antibody in Immunoprecipitation (IP) — Immunoprecipitation of Heat Shock Protein 90 beta using Heat Shock Protein 90 beta Polyclonal Antibody (Product # PA5-16327) on Native Mouse MAD109 Cells.

Product Details

PA5-16327

Applications	Tested Dilution	Publications
Western Blot (WB)	1 µg/mL	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1:100-1:500	View 1 publication 1 publication
Immunoprecipitation (IP)	2 µg/mL	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Pig, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthetic peptide corresponding to aa 2-13 (PEEVHHGEEEVE-Cys) from the N-terminus end of mouse heat shock protein 90beta View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_10976835

Product Specific Information

PA5-16327 targets Heat Shock Protein 90 beta in IP, IF/ICC, and WB applications and shows reactivity with mouse, Rat, and Human samples.

The PA5-16327 immunogen is a synthetic peptiude corresponding to aa 2-13 (PEEVHHGEEEVE-Cys) from the N-terminus end of mouse heat shock protein 90beta.

This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

Target Information

Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, aryl hydrocarbon (Ah) receptor, and some kinases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Functional complexity of the axonal growth cone: a proteomic analysis.

PloS one

Estrada-Bernal A,Sanford SD,Sosa LJ,Simon GC,Hansen KC,Pfenninger KH

PA5-16327 was used in immunohistochemistry to study the proteomics of the axonal growth cone

Mon Aug 27 00:00:00 EDT 2012

Differential proteome and transcriptome analysis of porcine skeletal muscle during development.

Journal of proteomics

Xu Y,Qian H,Feng X,Xiong Y,Lei M,Ren Z,Zuo B,Xu D,Ma Y,Yuan H

PA5-16327 was used in western blot to study developmental changes in pig skeletal muscle at the transcriptomic and proteomic levels

Tue Apr 03 00:00:00 EDT 2012

Hectd1 regulates intracellular localization and secretion of Hsp90 to control cellular behavior of the cranial mesenchyme.

The Journal of cell biology

Sarkar AA,Zohn IE

PA5-16327 was used in immunocytochemistry, immunoprecipitation, and western blot to elucidate the molecular cause of the abnormal behavior of Hectd1 mutant cranial mesenchyme

Mon Mar 19 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: Heat shock 84 kDa; heat shock 90kD protein 1, beta; heat shock 90kDa protein 1, beta; Heat shock protein; heat shock protein 1, beta; heat shock protein 90 kDa; heat shock protein 90kDa alpha (cytosolic), class B member 1; heat shock protein 90kDa alpha family class B member 1; Heat shock protein HSP 90-beta; heat shock protein, 84 kDa 1; HSP; HSP 84; HSP 90; HSP84; HSP90-beta; retinal degeneration slow protein; RP1-302G2.1; TSTA; Tumor-specific transplantation 84 kDa antigen

Gene Aliases: 90kDa; AL022974; C81438; D6S182; HSP84; Hsp84-1; Hsp90; HSP90-BETA; HSP90AB1; HSP90B; HSPC2; HSPCB

UniProt ID: (Human) P08238, (Mouse) P11499, (Rat) P34058

Entrez Gene ID: (Human) 3326, (Mouse) 15516, (Rat) 301252

Molecular Function: Hsp90 family chaperone